Multiplex profiling of cellular invasion in 3D cell culture models.
To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces. Here, we describe a 96-well microplate-based, high-content, three-dimensional cell culture assay capable of assessi...
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doaj-1a4301e54d784a8c842c7f3cdbda79fa2021-03-03T20:23:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6312110.1371/journal.pone.0063121Multiplex profiling of cellular invasion in 3D cell culture models.Gerald BurgstallerBettina OehrleIna KochMichael LindnerOliver EickelbergTo-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces. Here, we describe a 96-well microplate-based, high-content, three-dimensional cell culture assay capable of assessing invasion dynamics and molecular signatures thereof. On applying our invasion assay, we were able to demonstrate significant effects on the invasion capacity of fibroblast cell lines, as well as primary lung fibroblasts. Administration of epidermal growth factor resulted in a substantial increase of cellular invasion, thus making this technique suitable for high-throughput pharmacological screening of novel compounds regulating invasive and migratory pathways of primary cells. Our assay also correlates cellular invasiveness to molecular events. Thus, we argue of having developed a powerful and versatile toolbox for an extensive profiling of invasive cells in a 96-well format. This will have a major impact on research in disease areas like fibrosis, metastatic cancers, or chronic inflammatory states.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23671660/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gerald Burgstaller Bettina Oehrle Ina Koch Michael Lindner Oliver Eickelberg |
spellingShingle |
Gerald Burgstaller Bettina Oehrle Ina Koch Michael Lindner Oliver Eickelberg Multiplex profiling of cellular invasion in 3D cell culture models. PLoS ONE |
author_facet |
Gerald Burgstaller Bettina Oehrle Ina Koch Michael Lindner Oliver Eickelberg |
author_sort |
Gerald Burgstaller |
title |
Multiplex profiling of cellular invasion in 3D cell culture models. |
title_short |
Multiplex profiling of cellular invasion in 3D cell culture models. |
title_full |
Multiplex profiling of cellular invasion in 3D cell culture models. |
title_fullStr |
Multiplex profiling of cellular invasion in 3D cell culture models. |
title_full_unstemmed |
Multiplex profiling of cellular invasion in 3D cell culture models. |
title_sort |
multiplex profiling of cellular invasion in 3d cell culture models. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces. Here, we describe a 96-well microplate-based, high-content, three-dimensional cell culture assay capable of assessing invasion dynamics and molecular signatures thereof. On applying our invasion assay, we were able to demonstrate significant effects on the invasion capacity of fibroblast cell lines, as well as primary lung fibroblasts. Administration of epidermal growth factor resulted in a substantial increase of cellular invasion, thus making this technique suitable for high-throughput pharmacological screening of novel compounds regulating invasive and migratory pathways of primary cells. Our assay also correlates cellular invasiveness to molecular events. Thus, we argue of having developed a powerful and versatile toolbox for an extensive profiling of invasive cells in a 96-well format. This will have a major impact on research in disease areas like fibrosis, metastatic cancers, or chronic inflammatory states. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23671660/?tool=EBI |
work_keys_str_mv |
AT geraldburgstaller multiplexprofilingofcellularinvasionin3dcellculturemodels AT bettinaoehrle multiplexprofilingofcellularinvasionin3dcellculturemodels AT inakoch multiplexprofilingofcellularinvasionin3dcellculturemodels AT michaellindner multiplexprofilingofcellularinvasionin3dcellculturemodels AT olivereickelberg multiplexprofilingofcellularinvasionin3dcellculturemodels |
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1714822707706593280 |