Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry
A reliable, sensitive and accurate multiple mycotoxin method was developed for the simultaneous determination of 17 mycotoxins in swine, poultry and dairy feeds using stable isotope dilution (<sup>13</sup>C-ISTD) and (ultra)-high performance liquid chromatography-tandem mass spectrometry...
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doaj-1a5bf1b264464df5aa453021af0e00792020-11-25T02:28:54ZengMDPI AGToxins2072-66512020-04-011225325310.3390/toxins12040253Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass SpectrometryKraiwut Nualkaw0Saranya Poapolathep1Zhaowei Zhang2Qi Zhang3Mario Giorgi4Peiwu Li5Antonio Francesco Logrieco6Amnart Poapolathep7Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, ThailandDepartment of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, ThailandOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaDepartment of Veterinary Science, University of Pisa, 56126 Pisa, ItalyOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaInstitute of Sciences of Food Production, National Research Council, 70126 Bari, ItalyDepartment of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, ThailandA reliable, sensitive and accurate multiple mycotoxin method was developed for the simultaneous determination of 17 mycotoxins in swine, poultry and dairy feeds using stable isotope dilution (<sup>13</sup>C-ISTD) and (ultra)-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A simple QuEChERS-based method (quick, easy, cheap, effective, rugged and safe) was developed consisting of soaking with a solution of 1% formic acid followed by extraction with acetonitrile, clean-up with C18 sorbent and finally adding <sup>13</sup>C-ISTD before the UHPLC-MS/MS analysis. The chromatographic condition was optimized for separation and detection of the 17 mycotoxins using gradient elution. The method’s performance complied with the SANTE/11813/2017 standard and had mean recovery accuracies in the range 70%–120% and precision testing of % relative standard deviation (RSD) £ 20%. The limit of detection and limit of quantification values ranged from 0.25 to 40.0 ng/g and 0.5 to 100.0 ng/g, respectively. Finally, the method was applied to analyze feed samples, with the results showing that fumonisins, zearalenone, aflatoxin B1 and deoxynivalenol were the most prevalent mycotoxins contaminating the feed samples.https://www.mdpi.com/2072-6651/12/4/253multi-mycotoxinsQuEChERSanimal feedsUHPLC-MS/MS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kraiwut Nualkaw Saranya Poapolathep Zhaowei Zhang Qi Zhang Mario Giorgi Peiwu Li Antonio Francesco Logrieco Amnart Poapolathep |
spellingShingle |
Kraiwut Nualkaw Saranya Poapolathep Zhaowei Zhang Qi Zhang Mario Giorgi Peiwu Li Antonio Francesco Logrieco Amnart Poapolathep Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry Toxins multi-mycotoxins QuEChERS animal feeds UHPLC-MS/MS |
author_facet |
Kraiwut Nualkaw Saranya Poapolathep Zhaowei Zhang Qi Zhang Mario Giorgi Peiwu Li Antonio Francesco Logrieco Amnart Poapolathep |
author_sort |
Kraiwut Nualkaw |
title |
Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_short |
Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_full |
Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_fullStr |
Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_full_unstemmed |
Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry |
title_sort |
simultaneous determination of multiple mycotoxins in swine, poultry and dairy feeds using ultra high performance liquid chromatography-tandem mass spectrometry |
publisher |
MDPI AG |
series |
Toxins |
issn |
2072-6651 |
publishDate |
2020-04-01 |
description |
A reliable, sensitive and accurate multiple mycotoxin method was developed for the simultaneous determination of 17 mycotoxins in swine, poultry and dairy feeds using stable isotope dilution (<sup>13</sup>C-ISTD) and (ultra)-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A simple QuEChERS-based method (quick, easy, cheap, effective, rugged and safe) was developed consisting of soaking with a solution of 1% formic acid followed by extraction with acetonitrile, clean-up with C18 sorbent and finally adding <sup>13</sup>C-ISTD before the UHPLC-MS/MS analysis. The chromatographic condition was optimized for separation and detection of the 17 mycotoxins using gradient elution. The method’s performance complied with the SANTE/11813/2017 standard and had mean recovery accuracies in the range 70%–120% and precision testing of % relative standard deviation (RSD) £ 20%. The limit of detection and limit of quantification values ranged from 0.25 to 40.0 ng/g and 0.5 to 100.0 ng/g, respectively. Finally, the method was applied to analyze feed samples, with the results showing that fumonisins, zearalenone, aflatoxin B1 and deoxynivalenol were the most prevalent mycotoxins contaminating the feed samples. |
topic |
multi-mycotoxins QuEChERS animal feeds UHPLC-MS/MS |
url |
https://www.mdpi.com/2072-6651/12/4/253 |
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