Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen

• Main Authors: Yue Xing, Xue-Ke Li, Si-Duo Lu, Jin Ma
• Format: Article
• Language: English
• Published: Press of International Journal of Ophthalmology (IJO PRESS) 2020-08-01
• Series: International Journal of Ophthalmology
• Subjects: opticin; collagen; retinal vascular endothelial cell; integrin; rhoa/rock1
• Online Access: http://ies.ijo.cn/en_publish/2020/8/20200805.pdf

AIM: To investigate the effects of collagen and opticin on the bioactivity of human retinal vascular endothelial cells (hRVECs), and explore its regulations by integrins and RhoA/ROCK1 signal pathway. METHODS: hRVECs were cultured in collagen and treated by opticin, and cell-based bioactivity assays of cell proliferation, migration, and adhesion were performed. The expression of integrin α2, integrin β1, RhoA and ROCK1 were examined with real-time PCR and Western blotting. RESULTS: Collagen could promote cell viability of proliferation and migration (all P<0.05), and enhance the mRNA expression of integrin α2, integrin β1, RhoA and ROCK1 (all P<0.05). Opticin could inhibit proliferation and migration ability of hRVECs cultured in collagen, and reduce the mRNA expression of integrin α2, integrin β1, RhoA and ROCK1 (all P<0.05). CONCLUSION: Collagen and opticin can affect bioactivity of hRVECs, which may be regulated by α2-, β1-integrins and RhoA/ROCK1 signal pathway.