Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as well as proteinase K digestion were compared with...

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Bibliographic Details
Main Authors: Tanja Hoffmann, Andreas Hahn, Jaco J. Verweij, Gérard Leboulle, Olfert Landt, Christina Strube, Simone Kann, Denise Dekker, Jürgen May, Hagen Frickmann, Ulrike Loderstädt
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Pathogens
Subjects:
helminths
stool samples
consensus
nematodes
trematodes
cestodes
Online Access:https://www.mdpi.com/2076-0817/10/2/188

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