Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion.
Ex vivo heart perfusion systems, allowing continuous perfusion of the coronary vasculature, have recently been introduced to limit ischemic time of donor hearts prior to transplantation. Hearts are, however, perfused in an unloaded manner (via the aorta) and therefore, cardiac contractile function c...
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doaj-1a761c94f4a3430fb13d42eed362fc0f2020-11-24T22:07:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01134e019572110.1371/journal.pone.0195721Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion.Emilie FarineManuel U EgleAlice C BooneSandro ChristensenThierry P CarrelHendrik T Tevaearai StahelSarah L LongnusEx vivo heart perfusion systems, allowing continuous perfusion of the coronary vasculature, have recently been introduced to limit ischemic time of donor hearts prior to transplantation. Hearts are, however, perfused in an unloaded manner (via the aorta) and therefore, cardiac contractile function cannot be reliably evaluated.We aim to develop a ventricular loading device that enables monitoring of myocardial function in an ex vivo perfusion system. In this initial study, was to develop a prototype for rat experimentation.We designed a device consisting of a ventricular balloon and a reservoir balloon, connected through an electronic check valve, which opens and closes in coordination with changes in ventricular pressure. All balloons were produced in our laboratory and their properties, particularly pressure-volume relationships, were characterized. We developed a mock ventricle in vitro test system to evaluate the device, which was ultimately tested in ex vivo perfused rat hearts.Balloon production was consistent and balloon properties were maintained over time and with use on the device. Results from in vitro and ex vivo experiments show that the device functions appropriately; hemodynamic function can be measured and compares well to measurements made in an isolated, working (loaded) rat heart preparation.Our cardiac loading device appears to reliably allow measurement of several left ventricular hemodynamic parameters and provides the opportunity to control ventricular load.http://europepmc.org/articles/PMC5922540?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emilie Farine Manuel U Egle Alice C Boone Sandro Christensen Thierry P Carrel Hendrik T Tevaearai Stahel Sarah L Longnus |
spellingShingle |
Emilie Farine Manuel U Egle Alice C Boone Sandro Christensen Thierry P Carrel Hendrik T Tevaearai Stahel Sarah L Longnus Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. PLoS ONE |
author_facet |
Emilie Farine Manuel U Egle Alice C Boone Sandro Christensen Thierry P Carrel Hendrik T Tevaearai Stahel Sarah L Longnus |
author_sort |
Emilie Farine |
title |
Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. |
title_short |
Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. |
title_full |
Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. |
title_fullStr |
Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. |
title_full_unstemmed |
Development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. |
title_sort |
development of a cardiac loading device to monitor cardiac function during ex vivo graft perfusion. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
Ex vivo heart perfusion systems, allowing continuous perfusion of the coronary vasculature, have recently been introduced to limit ischemic time of donor hearts prior to transplantation. Hearts are, however, perfused in an unloaded manner (via the aorta) and therefore, cardiac contractile function cannot be reliably evaluated.We aim to develop a ventricular loading device that enables monitoring of myocardial function in an ex vivo perfusion system. In this initial study, was to develop a prototype for rat experimentation.We designed a device consisting of a ventricular balloon and a reservoir balloon, connected through an electronic check valve, which opens and closes in coordination with changes in ventricular pressure. All balloons were produced in our laboratory and their properties, particularly pressure-volume relationships, were characterized. We developed a mock ventricle in vitro test system to evaluate the device, which was ultimately tested in ex vivo perfused rat hearts.Balloon production was consistent and balloon properties were maintained over time and with use on the device. Results from in vitro and ex vivo experiments show that the device functions appropriately; hemodynamic function can be measured and compares well to measurements made in an isolated, working (loaded) rat heart preparation.Our cardiac loading device appears to reliably allow measurement of several left ventricular hemodynamic parameters and provides the opportunity to control ventricular load. |
url |
http://europepmc.org/articles/PMC5922540?pdf=render |
work_keys_str_mv |
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