Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using <i>Citrobacter</i> spp. MS5 Culture Supernatant
The present study described the extracellular synthesis of silver nanoparticles (AgNPs) using environmental bacterial isolate <i>Citrobacter</i> spp. MS5 culture supernatant. To our best knowledge, no previous study reported the biosynthesis of AgNPs using this bacterial isolate. The bio...
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doaj-1a7c40e8447d4dabb65c7abda1921ebb2020-11-25T03:03:59ZengMDPI AGBiomolecules2218-273X2020-06-011094494410.3390/biom10060944Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using <i>Citrobacter</i> spp. MS5 Culture SupernatantAftab Hossain Mondal0Dhananjay Yadav1Asghar Ali2Neelofar Khan3Jun O Jin4Qazi Mohd Rizwanul Haq5Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749, KoreaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Medical Biotechnology, Yeungnam University, Gyeongsan, 712-749, KoreaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaThe present study described the extracellular synthesis of silver nanoparticles (AgNPs) using environmental bacterial isolate <i>Citrobacter</i> spp. MS5 culture supernatant. To our best knowledge, no previous study reported the biosynthesis of AgNPs using this bacterial isolate. The biosynthesized AgNPs were characterized using different techniques like UV-Vis spectroscopy, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) equipped with energy dispersive X-ray (EDX). The analysis of UV-Vis spectra revealed absorption maxima at 415 nm due to surface plasmon resonance (SPR) indicated the formation of AgNPs and FTIR spectrum confirmed the participation of proteins molecule in AgNPs synthesis. XRD and EDX spectrum confirmed the metallic and crystalline nature of AgNPs. TEM and SEM showed spherical nanoparticles with a size range of 5–15 nm. The biosynthesized AgNPs showed effective independent as well as enhanced combined antibacterial activity against extended spectrum β-lactamase (ESBL) producing multidrug resistant Gram-negative bacteria. Further, effective antifungal activity of AgNPs was observed towards pathogenic <i>Candida</i> spp. The present study provides evidence for eco-friendly biosynthesis of well-characterized AgNPs and their potential antibacterial as well as antifungal activity.https://www.mdpi.com/2218-273X/10/6/944silver nanoparticles<i>Citrobacter</i> spp. MS5Gram-negative bacteriaantibacterialantifungal |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aftab Hossain Mondal Dhananjay Yadav Asghar Ali Neelofar Khan Jun O Jin Qazi Mohd Rizwanul Haq |
spellingShingle |
Aftab Hossain Mondal Dhananjay Yadav Asghar Ali Neelofar Khan Jun O Jin Qazi Mohd Rizwanul Haq Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using <i>Citrobacter</i> spp. MS5 Culture Supernatant Biomolecules silver nanoparticles <i>Citrobacter</i> spp. MS5 Gram-negative bacteria antibacterial antifungal |
author_facet |
Aftab Hossain Mondal Dhananjay Yadav Asghar Ali Neelofar Khan Jun O Jin Qazi Mohd Rizwanul Haq |
author_sort |
Aftab Hossain Mondal |
title |
Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using <i>Citrobacter</i> spp. MS5 Culture Supernatant |
title_short |
Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using <i>Citrobacter</i> spp. MS5 Culture Supernatant |
title_full |
Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using <i>Citrobacter</i> spp. MS5 Culture Supernatant |
title_fullStr |
Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using <i>Citrobacter</i> spp. MS5 Culture Supernatant |
title_full_unstemmed |
Anti-Bacterial and Anti-Candidal Activity of Silver Nanoparticles Biosynthesized Using <i>Citrobacter</i> spp. MS5 Culture Supernatant |
title_sort |
anti-bacterial and anti-candidal activity of silver nanoparticles biosynthesized using <i>citrobacter</i> spp. ms5 culture supernatant |
publisher |
MDPI AG |
series |
Biomolecules |
issn |
2218-273X |
publishDate |
2020-06-01 |
description |
The present study described the extracellular synthesis of silver nanoparticles (AgNPs) using environmental bacterial isolate <i>Citrobacter</i> spp. MS5 culture supernatant. To our best knowledge, no previous study reported the biosynthesis of AgNPs using this bacterial isolate. The biosynthesized AgNPs were characterized using different techniques like UV-Vis spectroscopy, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) equipped with energy dispersive X-ray (EDX). The analysis of UV-Vis spectra revealed absorption maxima at 415 nm due to surface plasmon resonance (SPR) indicated the formation of AgNPs and FTIR spectrum confirmed the participation of proteins molecule in AgNPs synthesis. XRD and EDX spectrum confirmed the metallic and crystalline nature of AgNPs. TEM and SEM showed spherical nanoparticles with a size range of 5–15 nm. The biosynthesized AgNPs showed effective independent as well as enhanced combined antibacterial activity against extended spectrum β-lactamase (ESBL) producing multidrug resistant Gram-negative bacteria. Further, effective antifungal activity of AgNPs was observed towards pathogenic <i>Candida</i> spp. The present study provides evidence for eco-friendly biosynthesis of well-characterized AgNPs and their potential antibacterial as well as antifungal activity. |
topic |
silver nanoparticles <i>Citrobacter</i> spp. MS5 Gram-negative bacteria antibacterial antifungal |
url |
https://www.mdpi.com/2218-273X/10/6/944 |
work_keys_str_mv |
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