Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease
Human oral soft tissues provide the first barrier of defence against chronic inflammatory disease and hold a remarkable scarless wounding phenotype. Tissue homeostasis requires coordinated actions of epithelial, mesenchymal, and immune cells. However, the extent of heterogeneity within the human ora...
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doaj-1a805d7df6f44d0a9c381bcceb6a60832021-05-05T22:39:13ZengeLife Sciences Publications LtdeLife2050-084X2021-01-011010.7554/eLife.62810Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory diseaseAna J Caetano0https://orcid.org/0000-0003-4588-3241Val Yianni1https://orcid.org/0000-0001-9857-7577Ana Volponi2Veronica Booth3Eleanor M D'Agostino4Paul Sharpe5https://orcid.org/0000-0003-2116-9561Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London, United KingdomCentre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London, United KingdomCentre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London, United KingdomDepartment of Periodontology, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London, United KingdomUnilever R&D, Colworth Science Park, Sharnbrook, Bedfordshire, Bedford, United KingdomCentre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London, United KingdomHuman oral soft tissues provide the first barrier of defence against chronic inflammatory disease and hold a remarkable scarless wounding phenotype. Tissue homeostasis requires coordinated actions of epithelial, mesenchymal, and immune cells. However, the extent of heterogeneity within the human oral mucosa and how tissue cell types are affected during the course of disease progression is unknown. Using single-cell transcriptome profiling we reveal a striking remodelling of the epithelial and mesenchymal niches with a decrease in functional populations that are linked to the aetiology of the disease. Analysis of ligand–receptor interaction pairs identify potential intercellular hubs driving the inflammatory component of the disease. Our work establishes a reference map of the human oral mucosa in health and disease, and a framework for the development of new therapeutic strategies.https://elifesciences.org/articles/62810oral mucosaperiodontitisgingivatranscriptomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana J Caetano Val Yianni Ana Volponi Veronica Booth Eleanor M D'Agostino Paul Sharpe |
spellingShingle |
Ana J Caetano Val Yianni Ana Volponi Veronica Booth Eleanor M D'Agostino Paul Sharpe Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease eLife oral mucosa periodontitis gingiva transcriptomics |
author_facet |
Ana J Caetano Val Yianni Ana Volponi Veronica Booth Eleanor M D'Agostino Paul Sharpe |
author_sort |
Ana J Caetano |
title |
Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease |
title_short |
Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease |
title_full |
Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease |
title_fullStr |
Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease |
title_full_unstemmed |
Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease |
title_sort |
defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2021-01-01 |
description |
Human oral soft tissues provide the first barrier of defence against chronic inflammatory disease and hold a remarkable scarless wounding phenotype. Tissue homeostasis requires coordinated actions of epithelial, mesenchymal, and immune cells. However, the extent of heterogeneity within the human oral mucosa and how tissue cell types are affected during the course of disease progression is unknown. Using single-cell transcriptome profiling we reveal a striking remodelling of the epithelial and mesenchymal niches with a decrease in functional populations that are linked to the aetiology of the disease. Analysis of ligand–receptor interaction pairs identify potential intercellular hubs driving the inflammatory component of the disease. Our work establishes a reference map of the human oral mucosa in health and disease, and a framework for the development of new therapeutic strategies. |
topic |
oral mucosa periodontitis gingiva transcriptomics |
url |
https://elifesciences.org/articles/62810 |
work_keys_str_mv |
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