Signatures of B Cell Receptor Repertoire Following Pneumocystis Infection
B cells play vital roles in host defense against Pneumocystis infection. However, the features of the B cell receptor (BCR) repertoire in disease progression remain unclear. Here, we integrated single-cell RNA sequencing and single-cell BCR sequencing of immune cells from mouse lungs in an uninfecte...
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2021-05-01
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doaj-1a8400a8d4ba43049ae87739576afa5f2021-05-31T15:22:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-05-011210.3389/fmicb.2021.636250636250Signatures of B Cell Receptor Repertoire Following Pneumocystis InfectionHan SunHu-Qin YangKan ZhaiZhao-Hui TongB cells play vital roles in host defense against Pneumocystis infection. However, the features of the B cell receptor (BCR) repertoire in disease progression remain unclear. Here, we integrated single-cell RNA sequencing and single-cell BCR sequencing of immune cells from mouse lungs in an uninfected state and 1–4 weeks post-infection in order to illustrate the dynamic nature of B cell responses during Pneumocystis infection. We identified continuously increased plasma cells and an elevated ratio of (IgA + IgG) to (IgD + IgM) after infection. Moreover, Pneumocystis infection was associated with an increasing naïve B subset characterized by elevated expression of the transcription factor ATF3. The proportion of clonal expanded cells progressively increased, while BCR diversity decreased. Plasma cells exhibited higher levels of somatic hypermutation than naïve B cells. Biased usage of V(D)J genes was observed, and the usage frequency of IGHV9-3 rose. Overall, these results present a detailed atlas of B cell transcriptional changes and BCR repertoire features in the context of Pneumocystis infection, which provides valuable information for finding diagnostic biomarkers and developing potential immunotherapeutic targets.https://www.frontiersin.org/articles/10.3389/fmicb.2021.636250/fullPneumocystis infectionB cell receptorsingle-cell BCR sequencingsingle-cell RNA sequencingsomatic hypermutation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Han Sun Hu-Qin Yang Kan Zhai Zhao-Hui Tong |
spellingShingle |
Han Sun Hu-Qin Yang Kan Zhai Zhao-Hui Tong Signatures of B Cell Receptor Repertoire Following Pneumocystis Infection Frontiers in Microbiology Pneumocystis infection B cell receptor single-cell BCR sequencing single-cell RNA sequencing somatic hypermutation |
author_facet |
Han Sun Hu-Qin Yang Kan Zhai Zhao-Hui Tong |
author_sort |
Han Sun |
title |
Signatures of B Cell Receptor Repertoire Following Pneumocystis Infection |
title_short |
Signatures of B Cell Receptor Repertoire Following Pneumocystis Infection |
title_full |
Signatures of B Cell Receptor Repertoire Following Pneumocystis Infection |
title_fullStr |
Signatures of B Cell Receptor Repertoire Following Pneumocystis Infection |
title_full_unstemmed |
Signatures of B Cell Receptor Repertoire Following Pneumocystis Infection |
title_sort |
signatures of b cell receptor repertoire following pneumocystis infection |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-05-01 |
description |
B cells play vital roles in host defense against Pneumocystis infection. However, the features of the B cell receptor (BCR) repertoire in disease progression remain unclear. Here, we integrated single-cell RNA sequencing and single-cell BCR sequencing of immune cells from mouse lungs in an uninfected state and 1–4 weeks post-infection in order to illustrate the dynamic nature of B cell responses during Pneumocystis infection. We identified continuously increased plasma cells and an elevated ratio of (IgA + IgG) to (IgD + IgM) after infection. Moreover, Pneumocystis infection was associated with an increasing naïve B subset characterized by elevated expression of the transcription factor ATF3. The proportion of clonal expanded cells progressively increased, while BCR diversity decreased. Plasma cells exhibited higher levels of somatic hypermutation than naïve B cells. Biased usage of V(D)J genes was observed, and the usage frequency of IGHV9-3 rose. Overall, these results present a detailed atlas of B cell transcriptional changes and BCR repertoire features in the context of Pneumocystis infection, which provides valuable information for finding diagnostic biomarkers and developing potential immunotherapeutic targets. |
topic |
Pneumocystis infection B cell receptor single-cell BCR sequencing single-cell RNA sequencing somatic hypermutation |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.636250/full |
work_keys_str_mv |
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