High Level Resistance of Enterococcus faecium and E. faecalis Isolates from Municipal Sewage Treatment Plants to Gentamicin
Background: Enterococci are members of the normal gut flora and released into the environment via sewage outlets, where they can survive for long times. Infections with high-level gentamicin resistant (HLGR) enterococci are emerg­ing worldwide. HLGR enterococci have developed a resistanc...
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Tehran University of Medical Sciences
2008-05-01
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doaj-1ab5c8de36fb48c7a62360315b86ec822020-12-02T02:20:09ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852008-05-01371103107High Level Resistance of Enterococcus faecium and E. faecalis Isolates from Municipal Sewage Treatment Plants to GentamicinM SaifiMM Soltan DallalMR PourshafieMR EshraghianMR PourmandMH SalariMH ShiraziBackground: Enterococci are members of the normal gut flora and released into the environment via sewage outlets, where they can survive for long times. Infections with high-level gentamicin resistant (HLGR) enterococci are emerg­ing worldwide. HLGR enterococci have developed a resistance to most antibiotics commonly used for enterococcal in­fec­tions therefore; treatment of infections caused by HLGR enterococci is difficult. The present study investigated the dis­tribution and antibiotic resistance of HLGR Enterococcus faecium and E. faecalis isolates from raw wastewater sam­ples in Tehran.Methods: Raw wastewater samples were collected during the period from November 2006 to May 2007 at 3 sewage treat­ment plants located in different parts of Tehran. All 90 HLGR enterococcal isolates were identified to the species level by biochemical and PCR assays and subjected to antibiotic susceptibility testing. Results: Sixty four percent (58 of 90) of isolates were E. faecium and 29%(26 of 90) of them were E. faecalis. The high­est level of antibiotic resistance was observed with erythromycin (63%), co-trimoxazole (69%) and tetracycline (92%) for E.faecalis and with erythromycin (97%), ciprofloxacin (47%), co-trimoxazole (45.5%) and tetracycline (47%) for E. faecium. Multiresistance against 3 to 4 antimicrobial was present in 27.5% and 15.5% of the isolates, re­spectively. Conclusion: HLGR E. faecium were more commonly found than E. faecalis. Species identification of HLGR entero­cocci enables us to assess species-specific antibiotic susceptibility patterns in our area. The present study reviled that HLGR E. faecalis remained more susceptible than E. faecium against the usual first-line and alternative treatments.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/6092.pdf&manuscript_id=6092E. faecalisgentamicin resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M Saifi MM Soltan Dallal MR Pourshafie MR Eshraghian MR Pourmand MH Salari MH Shirazi |
spellingShingle |
M Saifi MM Soltan Dallal MR Pourshafie MR Eshraghian MR Pourmand MH Salari MH Shirazi High Level Resistance of Enterococcus faecium and E. faecalis Isolates from Municipal Sewage Treatment Plants to Gentamicin Iranian Journal of Public Health E. faecalis gentamicin resistance |
author_facet |
M Saifi MM Soltan Dallal MR Pourshafie MR Eshraghian MR Pourmand MH Salari MH Shirazi |
author_sort |
M Saifi |
title |
High Level Resistance of Enterococcus faecium and E. faecalis Isolates from Municipal Sewage Treatment Plants to Gentamicin |
title_short |
High Level Resistance of Enterococcus faecium and E. faecalis Isolates from Municipal Sewage Treatment Plants to Gentamicin |
title_full |
High Level Resistance of Enterococcus faecium and E. faecalis Isolates from Municipal Sewage Treatment Plants to Gentamicin |
title_fullStr |
High Level Resistance of Enterococcus faecium and E. faecalis Isolates from Municipal Sewage Treatment Plants to Gentamicin |
title_full_unstemmed |
High Level Resistance of Enterococcus faecium and E. faecalis Isolates from Municipal Sewage Treatment Plants to Gentamicin |
title_sort |
high level resistance of enterococcus faecium and e. faecalis isolates from municipal sewage treatment plants to gentamicin |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Public Health |
issn |
2251-6085 |
publishDate |
2008-05-01 |
description |
Background: Enterococci are members of the normal gut flora and released into the environment via sewage outlets, where they can survive for long times. Infections with high-level gentamicin resistant (HLGR) enterococci are emerg­ing worldwide. HLGR enterococci have developed a resistance to most antibiotics commonly used for enterococcal in­fec­tions therefore; treatment of infections caused by HLGR enterococci is difficult. The present study investigated the dis­tribution and antibiotic resistance of HLGR Enterococcus faecium and E. faecalis isolates from raw wastewater sam­ples in Tehran.Methods: Raw wastewater samples were collected during the period from November 2006 to May 2007 at 3 sewage treat­ment plants located in different parts of Tehran. All 90 HLGR enterococcal isolates were identified to the species level by biochemical and PCR assays and subjected to antibiotic susceptibility testing. Results: Sixty four percent (58 of 90) of isolates were E. faecium and 29%(26 of 90) of them were E. faecalis. The high­est level of antibiotic resistance was observed with erythromycin (63%), co-trimoxazole (69%) and tetracycline (92%) for E.faecalis and with erythromycin (97%), ciprofloxacin (47%), co-trimoxazole (45.5%) and tetracycline (47%) for E. faecium. Multiresistance against 3 to 4 antimicrobial was present in 27.5% and 15.5% of the isolates, re­spectively. Conclusion: HLGR E. faecium were more commonly found than E. faecalis. Species identification of HLGR entero­cocci enables us to assess species-specific antibiotic susceptibility patterns in our area. The present study reviled that HLGR E. faecalis remained more susceptible than E. faecium against the usual first-line and alternative treatments. |
topic |
E. faecalis gentamicin resistance |
url |
http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/6092.pdf&manuscript_id=6092 |
work_keys_str_mv |
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