Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus
Summary: Cranial motor nuclei in the brainstem innervate diverse types of head and neck muscles. Failure in establishing these neuromuscular connections causes congenital cranial dysinnervation disorders (CCDDs) characterized by abnormal craniofacial movements. However, mechanisms that link cranial...
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doaj-1af3426ed03743d9a8726ebc01a21fb72020-11-25T00:27:23ZengElsevierCell Reports2211-12472018-08-0124615621572Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens NucleusKazuhide Asakawa0Koichi Kawakami1Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Corresponding authorDivision of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, Shizuoka 411-8540, Japan; Corresponding authorSummary: Cranial motor nuclei in the brainstem innervate diverse types of head and neck muscles. Failure in establishing these neuromuscular connections causes congenital cranial dysinnervation disorders (CCDDs) characterized by abnormal craniofacial movements. However, mechanisms that link cranial motor nuclei to target muscles are poorly understood at the molecular level. Here, we report that protocadherin-mediated repulsion mediates neuromuscular connection in the ocular motor system in zebrafish. We identify pools of abducens motor neurons that are topographically arranged according to soma size and convergently innervate a single muscle. Disruptions of Duane retraction syndrome-associated transcription factors reveal that these neurons require Mafba/MAFB, but not Sall4/SALL4, for differentiation. Furthermore, genetic perturbations of Pcdh17/protocadherin-17 result in defective axon growth and soma clumping, thereby abolishing neuromuscular connectivity. Our results suggest that protocadherin-mediated repulsion forms the central topography and efferent projection pattern of the abducens nucleus following Mafba-dependent specification and imply potential involvement of protocadherins in CCDD etiology. : Cranial neuromuscular systems have been relatively unexplored, because of their restricted tissue accessibility. Using zebrafish, Asakawa and Kawakami develop a model system to analyze cranial neuromuscular connectivity and find that protocadherin-mediated repulsion plays crucial roles for connecting the abducens nucleus to the lateral rectus muscle. Keywords: mnr2b, pcdh17, mafb, sall4, zebrafish, DRS, strabismus, lateral rectus, rhombomere, motor neuronhttp://www.sciencedirect.com/science/article/pii/S2211124718311148 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kazuhide Asakawa Koichi Kawakami |
spellingShingle |
Kazuhide Asakawa Koichi Kawakami Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus Cell Reports |
author_facet |
Kazuhide Asakawa Koichi Kawakami |
author_sort |
Kazuhide Asakawa |
title |
Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus |
title_short |
Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus |
title_full |
Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus |
title_fullStr |
Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus |
title_full_unstemmed |
Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus |
title_sort |
protocadherin-mediated cell repulsion controls the central topography and efferent projections of the abducens nucleus |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2018-08-01 |
description |
Summary: Cranial motor nuclei in the brainstem innervate diverse types of head and neck muscles. Failure in establishing these neuromuscular connections causes congenital cranial dysinnervation disorders (CCDDs) characterized by abnormal craniofacial movements. However, mechanisms that link cranial motor nuclei to target muscles are poorly understood at the molecular level. Here, we report that protocadherin-mediated repulsion mediates neuromuscular connection in the ocular motor system in zebrafish. We identify pools of abducens motor neurons that are topographically arranged according to soma size and convergently innervate a single muscle. Disruptions of Duane retraction syndrome-associated transcription factors reveal that these neurons require Mafba/MAFB, but not Sall4/SALL4, for differentiation. Furthermore, genetic perturbations of Pcdh17/protocadherin-17 result in defective axon growth and soma clumping, thereby abolishing neuromuscular connectivity. Our results suggest that protocadherin-mediated repulsion forms the central topography and efferent projection pattern of the abducens nucleus following Mafba-dependent specification and imply potential involvement of protocadherins in CCDD etiology. : Cranial neuromuscular systems have been relatively unexplored, because of their restricted tissue accessibility. Using zebrafish, Asakawa and Kawakami develop a model system to analyze cranial neuromuscular connectivity and find that protocadherin-mediated repulsion plays crucial roles for connecting the abducens nucleus to the lateral rectus muscle. Keywords: mnr2b, pcdh17, mafb, sall4, zebrafish, DRS, strabismus, lateral rectus, rhombomere, motor neuron |
url |
http://www.sciencedirect.com/science/article/pii/S2211124718311148 |
work_keys_str_mv |
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