Genotypic Characterization of Clinical Isolates of <i>Staphylococcus aureus</i> from Pakistan
In this study, we compared pulsed-field gel electrophoretic (PFGE), multilocus sequence typing (MLST), Staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>), <i>spa</i> typing, and virulence gene profiles of 19 Panton–Valentine leucocidin (PVL)-positive, m...
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doaj-1b04255147dc4816942205acf664abd92021-08-26T14:11:27ZengMDPI AGPathogens2076-08172021-07-011091891810.3390/pathogens10080918Genotypic Characterization of Clinical Isolates of <i>Staphylococcus aureus</i> from PakistanSaeed Khan0Bernard S. Marasa1Kidon Sung2Mohamed Nawaz3Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USADivision of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USAIn this study, we compared pulsed-field gel electrophoretic (PFGE), multilocus sequence typing (MLST), Staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>), <i>spa</i> typing, and virulence gene profiles of 19 Panton–Valentine leucocidin (PVL)-positive, multidrug-, and methicillin-resistant clinical <i>Staphylococcus aureus</i> (MRSA) isolates obtained from a hospital intensive care unit in Pakistan. The isolates exhibited 10 pulsotypes, contained eight adhesin genes (<i>bbp, clfA, clfB, cna, fnbA, fnbB, map-eap,</i> and <i>spa</i>), 10 toxin genes (<i>hla, hlb, hld, hlg, pvl, sed, see, seg, seh,</i> and <i>tst</i>), and two other virulence genes (<i>cfb, v8</i>) that were commonly present in all isolates. The <i>spa</i>-typing indicated seven known <i>spa</i> types (t030, t064, t138, t314, t987, t1509, and t5414) and three novel <i>spa</i> types. MLST analysis indicated eight ST types (ST8, ST15, ST30, ST239, ST291, ST503, ST772, and ST1413). All isolates belonged to the <i>agr</i> group 1. Most of the isolates possessed SCC<i>mec</i> type III, but some isolates had it in combination with types SCC<i>mec</i> IV and V. The presence of multidrug-resistant MRSA isolates in Pakistan indicates poor hygienic conditions, overuse of antibiotics, and a lack of rational antibiotic therapy that have led to the evolution and development of hypervirulent MRSA clones. The study warrants development of a robust epidemiological screening program and adoption of effective measures to stop their spread in hospitals and the community.https://www.mdpi.com/2076-0817/10/8/918methicillin resistancevirulencegenesMLSTSCC<i>mec</i><i>Staphylococcus aureus</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Saeed Khan Bernard S. Marasa Kidon Sung Mohamed Nawaz |
spellingShingle |
Saeed Khan Bernard S. Marasa Kidon Sung Mohamed Nawaz Genotypic Characterization of Clinical Isolates of <i>Staphylococcus aureus</i> from Pakistan Pathogens methicillin resistance virulence genes MLST SCC<i>mec</i> <i>Staphylococcus aureus</i> |
author_facet |
Saeed Khan Bernard S. Marasa Kidon Sung Mohamed Nawaz |
author_sort |
Saeed Khan |
title |
Genotypic Characterization of Clinical Isolates of <i>Staphylococcus aureus</i> from Pakistan |
title_short |
Genotypic Characterization of Clinical Isolates of <i>Staphylococcus aureus</i> from Pakistan |
title_full |
Genotypic Characterization of Clinical Isolates of <i>Staphylococcus aureus</i> from Pakistan |
title_fullStr |
Genotypic Characterization of Clinical Isolates of <i>Staphylococcus aureus</i> from Pakistan |
title_full_unstemmed |
Genotypic Characterization of Clinical Isolates of <i>Staphylococcus aureus</i> from Pakistan |
title_sort |
genotypic characterization of clinical isolates of <i>staphylococcus aureus</i> from pakistan |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2021-07-01 |
description |
In this study, we compared pulsed-field gel electrophoretic (PFGE), multilocus sequence typing (MLST), Staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>), <i>spa</i> typing, and virulence gene profiles of 19 Panton–Valentine leucocidin (PVL)-positive, multidrug-, and methicillin-resistant clinical <i>Staphylococcus aureus</i> (MRSA) isolates obtained from a hospital intensive care unit in Pakistan. The isolates exhibited 10 pulsotypes, contained eight adhesin genes (<i>bbp, clfA, clfB, cna, fnbA, fnbB, map-eap,</i> and <i>spa</i>), 10 toxin genes (<i>hla, hlb, hld, hlg, pvl, sed, see, seg, seh,</i> and <i>tst</i>), and two other virulence genes (<i>cfb, v8</i>) that were commonly present in all isolates. The <i>spa</i>-typing indicated seven known <i>spa</i> types (t030, t064, t138, t314, t987, t1509, and t5414) and three novel <i>spa</i> types. MLST analysis indicated eight ST types (ST8, ST15, ST30, ST239, ST291, ST503, ST772, and ST1413). All isolates belonged to the <i>agr</i> group 1. Most of the isolates possessed SCC<i>mec</i> type III, but some isolates had it in combination with types SCC<i>mec</i> IV and V. The presence of multidrug-resistant MRSA isolates in Pakistan indicates poor hygienic conditions, overuse of antibiotics, and a lack of rational antibiotic therapy that have led to the evolution and development of hypervirulent MRSA clones. The study warrants development of a robust epidemiological screening program and adoption of effective measures to stop their spread in hospitals and the community. |
topic |
methicillin resistance virulence genes MLST SCC<i>mec</i> <i>Staphylococcus aureus</i> |
url |
https://www.mdpi.com/2076-0817/10/8/918 |
work_keys_str_mv |
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1721190825429303296 |