Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids

Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids

Summary: Healthy vaginal epithelium is essential for normal reproductive functions and protects against infectious diseases. Here, we present a protocol for developing mouse vaginal organoids from single epithelial cells. These organoids recapitulate both functional and structural characteristics of...

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Main Authors: Ayesha Ali, Shafiq M. Syed, Pradeep S. Tanwar
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300757
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spelling doaj-1b17c2ce5e294bb79bda7f7a7d9eb8a62020-11-25T04:08:54ZengElsevierSTAR Protocols2666-16672020-09-0112100088Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal OrganoidsAyesha Ali0Shafiq M. Syed1Pradeep S. Tanwar2School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia; Hunter Medical Research Institute, Newcastle, NSW, Australia; Corresponding authorSchool of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia; Hunter Medical Research Institute, Newcastle, NSW, AustraliaSchool of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia; Hunter Medical Research Institute, Newcastle, NSW, Australia; Corresponding authorSummary: Healthy vaginal epithelium is essential for normal reproductive functions and protects against infectious diseases. Here, we present a protocol for developing mouse vaginal organoids from single epithelial cells. These organoids recapitulate both functional and structural characteristics of vagina in situ. This model is a powerful tool for investigating how vaginal microbiome or chemicals in contraceptives and personal hygiene products interact with stem cells and alter the epithelial dynamics, which will lead to new insights into the pathogenesis of vaginal diseases.For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720300757
collection DOAJ
language English
format Article
sources DOAJ
author Ayesha Ali
Shafiq M. Syed
Pradeep S. Tanwar
spellingShingle Ayesha Ali
Shafiq M. Syed
Pradeep S. Tanwar
Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids
STAR Protocols
author_facet Ayesha Ali
Shafiq M. Syed
Pradeep S. Tanwar
author_sort Ayesha Ali
title Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids
title_short Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids
title_full Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids
title_fullStr Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids
title_full_unstemmed Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids
title_sort protocol for in vitro establishment and long-term culture of mouse vaginal organoids
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-09-01
description Summary: Healthy vaginal epithelium is essential for normal reproductive functions and protects against infectious diseases. Here, we present a protocol for developing mouse vaginal organoids from single epithelial cells. These organoids recapitulate both functional and structural characteristics of vagina in situ. This model is a powerful tool for investigating how vaginal microbiome or chemicals in contraceptives and personal hygiene products interact with stem cells and alter the epithelial dynamics, which will lead to new insights into the pathogenesis of vaginal diseases.For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).
url http://www.sciencedirect.com/science/article/pii/S2666166720300757
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AT shafiqmsyed protocolforinvitroestablishmentandlongtermcultureofmousevaginalorganoids
AT pradeepstanwar protocolforinvitroestablishmentandlongtermcultureofmousevaginalorganoids
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