A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences
Phenotypic complementation of gene knockouts is an essential step in establishing function. Here, we describe a simple strategy for ‘gold standard’ complementation in which the mutant allele is replaced in situ with a wild type (WT) allele in a procedure that exploits CRISPR/Cas9. The method relies...
Main Authors: | François M. Seys, Peter Rowe, Edward L. Bolt, Christopher M. Humphreys, Nigel P. Minton |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-04-01
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Series: | Genes |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4425/11/4/458 |
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