Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex
Abstract Genetic evidence indicates that haploinsufficiency of ARID1B causes intellectual disability (ID) and autism spectrum disorder (ASD), but the neural function of ARID1B is largely unknown. Using both conditional and global Arid1b knockout mouse strains, we examined the role of ARID1B in neura...
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2021-02-01
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doaj-1b26474e510c494a973e8f34bf106e312021-02-21T12:32:26ZengNature Publishing GroupScientific Reports2045-23222021-02-0111111710.1038/s41598-021-82974-yDifferential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortexJeffrey J. Moffat0Eui-Man Jung1Minhan Ka2Byeong Tak Jeon3Hyunkyoung Lee4Woo-Yang Kim5Developmental Neuroscience, University of Nebraska Medical CenterDepartment of Molecular Biology, Pusan National UniversityResearch Center for Substance Abuse Pharmacology, Korea Institute of ToxicologyDepartment of Biological Sciences, Kent State UniversityDepartment of Biological Sciences, Kent State UniversityDepartment of Biological Sciences, Kent State UniversityAbstract Genetic evidence indicates that haploinsufficiency of ARID1B causes intellectual disability (ID) and autism spectrum disorder (ASD), but the neural function of ARID1B is largely unknown. Using both conditional and global Arid1b knockout mouse strains, we examined the role of ARID1B in neural progenitors. We detected an overall decrease in the proliferation of cortical and ventral neural progenitors following homozygous deletion of Arid1b, as well as altered cell cycle regulation and increased cell death. Each of these phenotypes was more pronounced in ventral neural progenitors. Furthermore, we observed decreased nuclear localization of β-catenin in Arid1b-deficient neurons. Conditional homozygous deletion of Arid1b in ventral neural progenitors led to pronounced ID- and ASD-like behaviors in mice, whereas the deletion in cortical neural progenitors resulted in minor cognitive deficits. This study suggests an essential role for ARID1B in forebrain neurogenesis and clarifies its more pronounced role in inhibitory neural progenitors. Our findings also provide insights into the pathogenesis of ID and ASD.https://doi.org/10.1038/s41598-021-82974-y |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeffrey J. Moffat Eui-Man Jung Minhan Ka Byeong Tak Jeon Hyunkyoung Lee Woo-Yang Kim |
spellingShingle |
Jeffrey J. Moffat Eui-Man Jung Minhan Ka Byeong Tak Jeon Hyunkyoung Lee Woo-Yang Kim Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex Scientific Reports |
author_facet |
Jeffrey J. Moffat Eui-Man Jung Minhan Ka Byeong Tak Jeon Hyunkyoung Lee Woo-Yang Kim |
author_sort |
Jeffrey J. Moffat |
title |
Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex |
title_short |
Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex |
title_full |
Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex |
title_fullStr |
Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex |
title_full_unstemmed |
Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex |
title_sort |
differential roles of arid1b in excitatory and inhibitory neural progenitors in the developing cortex |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-02-01 |
description |
Abstract Genetic evidence indicates that haploinsufficiency of ARID1B causes intellectual disability (ID) and autism spectrum disorder (ASD), but the neural function of ARID1B is largely unknown. Using both conditional and global Arid1b knockout mouse strains, we examined the role of ARID1B in neural progenitors. We detected an overall decrease in the proliferation of cortical and ventral neural progenitors following homozygous deletion of Arid1b, as well as altered cell cycle regulation and increased cell death. Each of these phenotypes was more pronounced in ventral neural progenitors. Furthermore, we observed decreased nuclear localization of β-catenin in Arid1b-deficient neurons. Conditional homozygous deletion of Arid1b in ventral neural progenitors led to pronounced ID- and ASD-like behaviors in mice, whereas the deletion in cortical neural progenitors resulted in minor cognitive deficits. This study suggests an essential role for ARID1B in forebrain neurogenesis and clarifies its more pronounced role in inhibitory neural progenitors. Our findings also provide insights into the pathogenesis of ID and ASD. |
url |
https://doi.org/10.1038/s41598-021-82974-y |
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