Tracking single particles for hours via continuous DNA-mediated fluorophore exchange

The length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.

Bibliographic Details
Main Authors:	Florian Stehr, Johannes Stein, Julian Bauer, Christian Niederauer, Ralf Jungmann, Kristina Ganzinger, Petra Schwille
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-24223-4

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spelling	doaj-1b2f7a94852a468ebfe04d98c2bcdb3f2021-07-25T11:38:31ZengNature Publishing GroupNature Communications2041-17232021-07-011211810.1038/s41467-021-24223-4Tracking single particles for hours via continuous DNA-mediated fluorophore exchangeFlorian Stehr0Johannes Stein1Julian Bauer2Christian Niederauer3Ralf Jungmann4Kristina Ganzinger5Petra Schwille6Max Planck Institute of BiochemistryMax Planck Institute of BiochemistryMax Planck Institute of BiochemistryAMOLFMax Planck Institute of BiochemistryAMOLFMax Planck Institute of BiochemistryThe length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.https://doi.org/10.1038/s41467-021-24223-4
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Florian Stehr Johannes Stein Julian Bauer Christian Niederauer Ralf Jungmann Kristina Ganzinger Petra Schwille
spellingShingle	Florian Stehr Johannes Stein Julian Bauer Christian Niederauer Ralf Jungmann Kristina Ganzinger Petra Schwille Tracking single particles for hours via continuous DNA-mediated fluorophore exchange Nature Communications
author_facet	Florian Stehr Johannes Stein Julian Bauer Christian Niederauer Ralf Jungmann Kristina Ganzinger Petra Schwille
author_sort	Florian Stehr
title	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_short	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_full	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_fullStr	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_full_unstemmed	Tracking single particles for hours via continuous DNA-mediated fluorophore exchange
title_sort	tracking single particles for hours via continuous dna-mediated fluorophore exchange
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-07-01
description	The length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.
url	https://doi.org/10.1038/s41467-021-24223-4
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Tracking single particles for hours via continuous DNA-mediated fluorophore exchange

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