Silver nanoparticles-decorated reduced graphene oxide: A novel peroxidase-like activity nanomaterial for development of a colorimetric glucose biosensor

• Main Authors: Hoang V. Tran, Nghia D. Nguyen, Chinh T.Q. Tran, Luyen T. Tran, Thu D. Le, Huyen T.T. Tran, B. Piro, Chinh D. Huynh, Thinh N. Nguyen, Nga T.T. Nguyen, Hue T.M. Dang, Huy L. Nguyen, Lam D. Tran, Nghia T. Phan
• Format: Article
• Language: English
• Published: Elsevier 2020-07-01
• Series: Arabian Journal of Chemistry
• Subjects: Silver nanoparticles-reduced graphene oxide (AgNPs/rGO) nanocomposite; Peroxidase-like activity; Hydrogen peroxide (H2O2); Glucose Biosensor; Colorimetric assay; Glucose Oxidase (GOx)
• Online Access: http://www.sciencedirect.com/science/article/pii/S1878535220301556

In this study, we present a novel approach to prepare of a colorimetric chemical sensor for H2O2 and a glucose biosensor basing on the use of peroxidase-like activity of silver nanoparticles decorated on reduced graphene oxide sheets (AgNPs@rGO) nanocomposite. Herein, AgNPs@rGO nanocomposite was synthesized by a one-step hydrothermal reducing method and its physico-chemical properties were characterized by X-ray diffraction (XRD), Transmission Electron Microscopy (TEM), Ultraviolet–visible spectroscopy (UV–Vis), Fourier-Transform Infrared spectroscopy (FT-IR) and Energy Dispersive X-ray spectroscopy (EDX). Obtained evaluation results shown that the synthesized AgNPs/rGO nanocomposite has performed an efficient peroxidase-like activity for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMBred) by H2O2, leading to the oxidized form (TMBox) which presents a typical blue color (maximum of absorbance at λmax = 655 nm). A colorimetric assay for H2O2 detection was designed and fabricated with a limit of detection of 20 μM. Moreover, we have used of AgNPs/rGO nanocomposite combining with glucose oxidase (GOx) to develop of a colorimetric glucose biosensor with a low limit of detection of 40 μM and a linear dynamic range from 125 μM to 1 mM. This glucose test was applied to the detection of glucose in human serum samples.