Antimicrobial Activity of Bacillus sp. Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria
Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against...
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doaj-1b444d33df494505a9cbbec1513e4b522020-11-25T02:30:57ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062012-01-015012531Antimicrobial Activity of Bacillus sp. Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic BacteriaTanja Berić0Milan Kojić1Slaviša Stanković2Ljubiša Topisirović3Giuliano Degrassi4Michael Myers5Vittorio Venturi6Djordje Fira7Faculty of Biology, University of Belgrade, Studentski trg 16, RS-11010 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, RS-11010 Belgrade, SerbiaFaculty of Biology, University of Belgrade, Studentski trg 16, RS-11010 Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, RS-11010 Belgrade, SerbiaInternational Centre for Genetic Engineering and Biotechnology, AREA Science Park, Padriciano 99, IT-34149 Trieste, ItalyInternational Centre for Genetic Engineering and Biotechnology, AREA Science Park, Padriciano 99, IT-34149 Trieste, ItalyInternational Centre for Genetic Engineering and Biotechnology, AREA Science Park, Padriciano 99, IT-34149 Trieste, ItalyFaculty of Biology, University of Belgrade, Studentski trg 16, RS-11010 Belgrade, SerbiaScreening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae.http://hrcak.srce.hr/file/117223Bacillus sp.Xanthomonas oryzae pv. oryzaelipopeptidesiturinbacteriocin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tanja Berić Milan Kojić Slaviša Stanković Ljubiša Topisirović Giuliano Degrassi Michael Myers Vittorio Venturi Djordje Fira |
spellingShingle |
Tanja Berić Milan Kojić Slaviša Stanković Ljubiša Topisirović Giuliano Degrassi Michael Myers Vittorio Venturi Djordje Fira Antimicrobial Activity of Bacillus sp. Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria Food Technology and Biotechnology Bacillus sp. Xanthomonas oryzae pv. oryzae lipopeptides iturin bacteriocin |
author_facet |
Tanja Berić Milan Kojić Slaviša Stanković Ljubiša Topisirović Giuliano Degrassi Michael Myers Vittorio Venturi Djordje Fira |
author_sort |
Tanja Berić |
title |
Antimicrobial Activity of Bacillus sp. Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria |
title_short |
Antimicrobial Activity of Bacillus sp. Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria |
title_full |
Antimicrobial Activity of Bacillus sp. Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria |
title_fullStr |
Antimicrobial Activity of Bacillus sp. Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria |
title_full_unstemmed |
Antimicrobial Activity of Bacillus sp. Natural Isolates and Their Potential Use in the Biocontrol of Phytopathogenic Bacteria |
title_sort |
antimicrobial activity of bacillus sp. natural isolates and their potential use in the biocontrol of phytopathogenic bacteria |
publisher |
University of Zagreb |
series |
Food Technology and Biotechnology |
issn |
1330-9862 1334-2606 |
publishDate |
2012-01-01 |
description |
Screening of 203 Bacillus sp. natural isolates for antimicrobial activity against phytopathogenic bacteria showed that 127 tested strains inhibit at least one sensitive strain, which illustrates their potential use as biocontrol agents. Among them, 104 isolates showed significant antagonism against Xanthomonas oryzae pv. oryzae, and only one of these (VPS50.2) synthesizes bacteriocin. An additional screening tested whether 51 isolates contained genes involved in the biosynthesis of lipopeptides of the iturin and surfactin classes. Results show that 33 isolates harbour the operon for iturin biosynthesis, and six of them carry the sfp gene, responsible for the biosynthesis of surfactin. Lipopeptide purification from the supernatant of isolate SS12.9 (identified as B. subtilis or B. amyloliquefaciens) was performed using ethyl acetate extraction, ultrafiltration and reversed phase HPLC. Mass spectrometry analysis confirmed that isolate SS12.9 produces a substance of the iturin class with potential for biocontrol of X. oryzae pv. oryzae. |
topic |
Bacillus sp. Xanthomonas oryzae pv. oryzae lipopeptides iturin bacteriocin |
url |
http://hrcak.srce.hr/file/117223 |
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