Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats
BACKGROUND Sleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expres...
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Faculty of Medicine Trisakti University
2015-12-01
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doaj-1b4989beb56841dea21e07307b8852a22020-11-25T03:11:14ZengFaculty of Medicine Trisakti UniversityUniversa Medicina1907-30622407-22302015-12-01342879510.18051/UnivMed.2015.v34.87-9517Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male ratsFitranto Arjadi0Ginus Partadireja1Lientje Setyawan Maurits2Mulyono Pangestu3Jenderal Soedirman UniversityGadjah mada UniversityGadjah Mada UniversityMonash Institute of Medical Research AustraliaBACKGROUND Sleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expression as parameters. The aim of this study was to observe testicular MDA level and caspase-3 expression treated with paradoxical sleep deprivation (PSD), immobilization, and footshock stress and to determine the stress model with a significant effect in white male rats (Rattus norvegicus) . METHODS This experimental randomized study of posttest only with control group design was conducted on 24 white male Wistar strain rats, randomly allocated into four treatment groups, i.e. control (K1) without any stress treatment, PSD (KII), immobilization (KIII), and footshock stress (KIV). Treatments were given for 25 days to produce chronic stress. Testicular MDA concentration was examined by the ELISA method while caspase-3 was examined by the TUNEL method. RESULTS Mean testicular MDA concentration with one-way ANOVA test showed differences in means between the groups (p=0.000) and post hoc Tukey-HSD test showed significant results between PSD stress group versus control, immobilization and footshock stress groups. One-way ANOVA test showed a significant difference in caspase-3 expression in at least two treatment groups (p=0.008) and post-hoc Tuckey-LSD test showed significant differences between controls and all stress groups. CONCLUSION Sleep deprivation is a type of stress inducing changes in testicular MDA concentration and caspase-3 expression in male rat testes.https://univmed.org/ejurnal/index.php/medicina/article/view/18caspase-3 expressionstresstesticular malondialdehydemale rat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fitranto Arjadi Ginus Partadireja Lientje Setyawan Maurits Mulyono Pangestu |
spellingShingle |
Fitranto Arjadi Ginus Partadireja Lientje Setyawan Maurits Mulyono Pangestu Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats Universa Medicina caspase-3 expression stress testicular malondialdehyde male rat |
author_facet |
Fitranto Arjadi Ginus Partadireja Lientje Setyawan Maurits Mulyono Pangestu |
author_sort |
Fitranto Arjadi |
title |
Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats |
title_short |
Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats |
title_full |
Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats |
title_fullStr |
Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats |
title_full_unstemmed |
Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats |
title_sort |
paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats |
publisher |
Faculty of Medicine Trisakti University |
series |
Universa Medicina |
issn |
1907-3062 2407-2230 |
publishDate |
2015-12-01 |
description |
BACKGROUND
Sleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expression as parameters. The aim of this study was to observe testicular MDA level and caspase-3 expression treated with paradoxical sleep deprivation (PSD), immobilization, and footshock stress and to determine the stress model with a significant effect in white male rats (Rattus norvegicus) .
METHODS
This experimental randomized study of posttest only with control group design was conducted on 24 white male Wistar strain rats, randomly allocated into four treatment groups, i.e. control (K1) without any stress treatment, PSD (KII), immobilization (KIII), and footshock stress (KIV). Treatments were given for 25 days to produce chronic stress. Testicular MDA concentration was examined by the ELISA method while caspase-3 was examined by the TUNEL method.
RESULTS
Mean testicular MDA concentration with one-way ANOVA test showed differences in means between the groups (p=0.000) and post hoc Tukey-HSD test showed significant results between PSD stress group versus control, immobilization and footshock stress groups. One-way ANOVA test showed a significant difference in caspase-3 expression in at least two treatment groups (p=0.008) and post-hoc Tuckey-LSD test showed significant differences between controls and all stress groups.
CONCLUSION
Sleep deprivation is a type of stress inducing changes in testicular MDA concentration and caspase-3 expression in male rat testes. |
topic |
caspase-3 expression stress testicular malondialdehyde male rat |
url |
https://univmed.org/ejurnal/index.php/medicina/article/view/18 |
work_keys_str_mv |
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