Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens
Objective: The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. Method: AFB smear microscopy, DTB and cultur...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2012-05-01
|
Series: | Brazilian Journal of Infectious Diseases |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867012703185 |
id |
doaj-1b4cba6e94b34b99814ffadca5f80219 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hung-Chang Hung Chi-Ho Chan Shih-Ming Tsao Shiuan-Chih Chen Tzu-Chin Wu Min-Chi Lu Der-Jinn Wu Shu-Ju Huang Yuan-Ti Lee |
spellingShingle |
Hung-Chang Hung Chi-Ho Chan Shih-Ming Tsao Shiuan-Chih Chen Tzu-Chin Wu Min-Chi Lu Der-Jinn Wu Shu-Ju Huang Yuan-Ti Lee Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens Brazilian Journal of Infectious Diseases |
author_facet |
Hung-Chang Hung Chi-Ho Chan Shih-Ming Tsao Shiuan-Chih Chen Tzu-Chin Wu Min-Chi Lu Der-Jinn Wu Shu-Ju Huang Yuan-Ti Lee |
author_sort |
Hung-Chang Hung |
title |
Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens |
title_short |
Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens |
title_full |
Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens |
title_fullStr |
Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens |
title_full_unstemmed |
Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens |
title_sort |
effectiveness of the bdprobetec et system for detection of mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens |
publisher |
Elsevier |
series |
Brazilian Journal of Infectious Diseases |
issn |
1413-8670 |
publishDate |
2012-05-01 |
description |
Objective: The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. Method: AFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards. Results: The sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively). The sensitivity and specificity of the DTB assay in detecting MTB in sputum samples was 77.2% and 100% compared to clinical diagnosis, while AFB smear had a sensitivity and specificity of 70.3% and 26.3%, respectively. Compared to culture, DTB had a sensitivity and specificity of 82.8% and 93.2%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 41.9% and 87.7%, respectively. Compared to clinical diagnosis, DTB had a sensitivity and specificity of 67.2% and 100%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 34.8% and 79.5%, respectively. Conclusions: The superior performance of the DTB assay relative to AFB smear microscopy makes it a valuable tool to enable early diagnosis of MTB, thereby improving patient care and reducing transmission. Keywords: Bronchoalveolar lavage, Mycobacterium tuberculosis, Acid-fast bacilli staining, Molecular diagnosis, BDProbeTEC ET system |
url |
http://www.sciencedirect.com/science/article/pii/S1413867012703185 |
work_keys_str_mv |
AT hungchanghung effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens AT chihochan effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens AT shihmingtsao effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens AT shiuanchihchen effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens AT tzuchinwu effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens AT minchilu effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens AT derjinnwu effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens AT shujuhuang effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens AT yuantilee effectivenessofthebdprobetecetsystemfordetectionofmycobacteriumtuberculosiscomplexinsputumandbronchoalveolarlavagespecimens |
_version_ |
1724466347867373568 |
spelling |
doaj-1b4cba6e94b34b99814ffadca5f802192020-11-25T03:56:13ZengElsevierBrazilian Journal of Infectious Diseases1413-86702012-05-01163242249Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimensHung-Chang Hung0Chi-Ho Chan1Shih-Ming Tsao2Shiuan-Chih Chen3Tzu-Chin Wu4Min-Chi Lu5Der-Jinn Wu6Shu-Ju Huang7Yuan-Ti Lee8Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Potz Hospital, Department of Health, Executive Yuan, Chiayi, TaiwanFaculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Microbiology and Immunology, Chung Shan Medical University, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Chest, Department of Internal Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Infectious Diseases, Department of Internal Medicine, Chung Shan Medical University, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Family and Community Medicine, Chung Shan Medical University, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Chest, Department of Internal Medicine, Chung Shan Medical University, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Infectious Diseases, Department of Internal Medicine, Chung Shan Medical University, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Cardiology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Infectious Diseases, Department of Internal Medicine, Chung Shan Medical University, Taichung, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Faculty of Medicine, School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Infectious Diseases, Department of Internal Medicine, Chung Shan Medical University, Taichung, Taiwan; Corresponding author at: Division of Infectious Diseases, Department of Internal Medicine, Chung Shan Medical University Hospital, No.110, Sec.1, Jianguo N. Rd., South District, Taichung City 40201, Taiwan.Objective: The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. Method: AFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards. Results: The sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively). The sensitivity and specificity of the DTB assay in detecting MTB in sputum samples was 77.2% and 100% compared to clinical diagnosis, while AFB smear had a sensitivity and specificity of 70.3% and 26.3%, respectively. Compared to culture, DTB had a sensitivity and specificity of 82.8% and 93.2%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 41.9% and 87.7%, respectively. Compared to clinical diagnosis, DTB had a sensitivity and specificity of 67.2% and 100%, respectively, in detecting MTB from BAL specimens; AFB smear had a sensitivity and specificity of 34.8% and 79.5%, respectively. Conclusions: The superior performance of the DTB assay relative to AFB smear microscopy makes it a valuable tool to enable early diagnosis of MTB, thereby improving patient care and reducing transmission. Keywords: Bronchoalveolar lavage, Mycobacterium tuberculosis, Acid-fast bacilli staining, Molecular diagnosis, BDProbeTEC ET systemhttp://www.sciencedirect.com/science/article/pii/S1413867012703185 |