The Effects of Turnip Mosaic Virus Infections on the Deposition of Secondary Cell Walls and Developmental Defects in Arabidopsis Plants Are Virus-Strain Specific
Two isolates of Turnip mosaic virus (UK 1 and JPN 1), representative of two different viral strains, induced differential alterations on secondary cell wall (SCW) development in Arabidopsis thaliana, suggesting cell-type specific effects of these viral infections. These potential effects were analyz...
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2021-10-01
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doaj-1b4d3c0cbc9d45a0a8f9bb3aad2e9af52021-10-08T07:38:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-10-011210.3389/fpls.2021.741050741050The Effects of Turnip Mosaic Virus Infections on the Deposition of Secondary Cell Walls and Developmental Defects in Arabidopsis Plants Are Virus-Strain SpecificSilvia López-González0Concepción Gómez-Mena1Flora Sánchez2Mathias Schuetz3A. Lacey Samuels4Fernando Ponz5Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, CSIC, Madrid, SpainInstituto de Biología Molecular y Celular de Plantas, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Valencia, SpainCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, CSIC, Madrid, SpainDepartment of Botany, University of British Columbia, Vancouver, BC, CanadaDepartment of Botany, University of British Columbia, Vancouver, BC, CanadaCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, CSIC, Madrid, SpainTwo isolates of Turnip mosaic virus (UK 1 and JPN 1), representative of two different viral strains, induced differential alterations on secondary cell wall (SCW) development in Arabidopsis thaliana, suggesting cell-type specific effects of these viral infections. These potential effects were analyzed in inflorescence stems and flowers of infected plants, together with other possible cellular effects of the infections. Results obtained from macroscopic and histochemical analyses showed that infection with either virus significantly narrowed stem area, but defects in SCW were only found in JPN 1 infections. In flowers, reduced endothecium lignification was also found for JPN 1, while UK 1 infections induced severe floral cell and organ development alterations. A transcriptomic analysis focused on genes controlling and regulating SCW formation also showed notable differences between both viral isolates. UK 1 infections induced a general transcriptional decrease of most regulatory genes, whereas a more complex pattern of alterations was found in JPN 1 infections. The role of the previously identified viral determinant of most developmental alterations, the P3 protein, was also studied through the use of viral chimeras. No SCW alterations or creeping habit growth were found in infections by the chimeras, indicating that if the P3 viral protein is involved in the determination of these symptoms, it is not the only determinant. Finally, considerations as to the possibility of a taxonomical reappraisal of these TuMV viral strains are provided.https://www.frontiersin.org/articles/10.3389/fpls.2021.741050/fullturnip mosaic virussecondary cell walldevelopmental alterationsviral strainsviral chimeras |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Silvia López-González Concepción Gómez-Mena Flora Sánchez Mathias Schuetz A. Lacey Samuels Fernando Ponz |
spellingShingle |
Silvia López-González Concepción Gómez-Mena Flora Sánchez Mathias Schuetz A. Lacey Samuels Fernando Ponz The Effects of Turnip Mosaic Virus Infections on the Deposition of Secondary Cell Walls and Developmental Defects in Arabidopsis Plants Are Virus-Strain Specific Frontiers in Plant Science turnip mosaic virus secondary cell wall developmental alterations viral strains viral chimeras |
author_facet |
Silvia López-González Concepción Gómez-Mena Flora Sánchez Mathias Schuetz A. Lacey Samuels Fernando Ponz |
author_sort |
Silvia López-González |
title |
The Effects of Turnip Mosaic Virus Infections on the Deposition of Secondary Cell Walls and Developmental Defects in Arabidopsis Plants Are Virus-Strain Specific |
title_short |
The Effects of Turnip Mosaic Virus Infections on the Deposition of Secondary Cell Walls and Developmental Defects in Arabidopsis Plants Are Virus-Strain Specific |
title_full |
The Effects of Turnip Mosaic Virus Infections on the Deposition of Secondary Cell Walls and Developmental Defects in Arabidopsis Plants Are Virus-Strain Specific |
title_fullStr |
The Effects of Turnip Mosaic Virus Infections on the Deposition of Secondary Cell Walls and Developmental Defects in Arabidopsis Plants Are Virus-Strain Specific |
title_full_unstemmed |
The Effects of Turnip Mosaic Virus Infections on the Deposition of Secondary Cell Walls and Developmental Defects in Arabidopsis Plants Are Virus-Strain Specific |
title_sort |
effects of turnip mosaic virus infections on the deposition of secondary cell walls and developmental defects in arabidopsis plants are virus-strain specific |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-10-01 |
description |
Two isolates of Turnip mosaic virus (UK 1 and JPN 1), representative of two different viral strains, induced differential alterations on secondary cell wall (SCW) development in Arabidopsis thaliana, suggesting cell-type specific effects of these viral infections. These potential effects were analyzed in inflorescence stems and flowers of infected plants, together with other possible cellular effects of the infections. Results obtained from macroscopic and histochemical analyses showed that infection with either virus significantly narrowed stem area, but defects in SCW were only found in JPN 1 infections. In flowers, reduced endothecium lignification was also found for JPN 1, while UK 1 infections induced severe floral cell and organ development alterations. A transcriptomic analysis focused on genes controlling and regulating SCW formation also showed notable differences between both viral isolates. UK 1 infections induced a general transcriptional decrease of most regulatory genes, whereas a more complex pattern of alterations was found in JPN 1 infections. The role of the previously identified viral determinant of most developmental alterations, the P3 protein, was also studied through the use of viral chimeras. No SCW alterations or creeping habit growth were found in infections by the chimeras, indicating that if the P3 viral protein is involved in the determination of these symptoms, it is not the only determinant. Finally, considerations as to the possibility of a taxonomical reappraisal of these TuMV viral strains are provided. |
topic |
turnip mosaic virus secondary cell wall developmental alterations viral strains viral chimeras |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.741050/full |
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