THE JOURNAL OF TROPICAL LIFE SCIENCE OPEN ACCESS Freely available online VOL. 5, NO. 2, pp. 88-91, May, 2015 Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L.

• Main Authors: Nina Artanti, Muhammad Hanafi, Rina Andriyani, Vienna Saraswati, Zalinar Udin, Puspa D. Lotulung, Ken Ichi Fujita, Yoshinosuke Usuki
• Format: Article
• Language: English
• Published: University of Brawijaya 2015-05-01
• Series: Journal of Tropical Life Science
• Subjects: asperuloside; Hedyotis corymbosa; HL60; KB; leaf; Rubiaceae; YMB-1
• Online Access: http://www.jtrolis.ub.ac.id/index.php/jtrolis/article/view/283/pdf_11

Hedyotis corymbosa L., with local name rumput mutiara, is an anti-inflammatory, anti-cancer and hepatoprotective traditional medicine. The ethanol extract of H. corymbosa L. shows inhibitory activity to humanYMB-1 breast cancer cell line with an IC50 of 6.51 μg/mL. The methylene chloride fraction shows a potential cytotoxic activity with an IC50 of 2.75 μg/mL. To obtain a lead compound, the extract was further purified by column chromatography. A pure compound is obtained which shows inhibitory activities against YMB-1, HL60 and KB human cell lines with IC50 values of 0.7; 11.0 and 104.2 μg/mL, respectively. Based on the 1D and 2D FT-NMR data, the isolated compound is an asperuloside.