Cacipacore virus as an emergent mosquito-borne Flavivirus

• Main Authors: Mario Luis Garcia de Figueiredo, Alberto Anastacio Amarilla, Glauciane Garcia de Figueiredo, Helda Liz Alfonso, Veronica Lippi, Felipe Gonçalves Motta Maia, Felipe Alves Morais, Cristóvão Alves da Costa, Dyana Alves Henriques, Edison Luis Durigon, Luiz Tadeu Moraes Figueiredo, Victor Hugo Aquino
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT)
• Series: Revista da Sociedade Brasileira de Medicina Tropical
• Subjects: Cacipacore vírus; Mosquitoes; Emerging Flavivirus
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000400539&lng=en&tlng=en

Abstract INTRODUCTION: Cacipacore virus (CPCV), a possible bird-associated flavivirus, has yet to be detected in mosquitoes. Our purpose is examining CPCV in mosquitoes from the Amazon region of Brazil. METHODS: Approximately 3,253 Culicidae (grouped into 264 pools) were collected from the Amazon region during 2002-2006 and analyzed using a Flavivirus genus-specific reverse transcription- polymerase chain reaction followed by nested polymerase chain reaction assay and by nucleotide sequencing of amplicons. RESULTS: Nucleotide sequences from five mosquito samples showed high similarity to the those of CPCV originally isolated in the Amazon region. CONCLUSIONS: This is the first report of CPCV-infected mosquitoes which has implications on the arbovirus maintenance in nature and transmission to man.