Analysis of LncRNA-mRNA Co-Expression Profiles in Patients With Polycystic Ovary Syndrome: A Pilot Study
PurposeThis study aimed to investigate the profiles of messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) in peripheral blood samples collected from polycystic ovary syndrome (PCOS) patients. In addition, an in-depth bioinformatics analysis regarding the lncRNA-mRNA co-expression network was p...
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doaj-1b95e6fd270e43dfaff349009314e5162021-04-14T06:20:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-04-011210.3389/fimmu.2021.669819669819Analysis of LncRNA-mRNA Co-Expression Profiles in Patients With Polycystic Ovary Syndrome: A Pilot StudyXiuhong Sun0Xiuhong Sun1Yishan Liu2Xinyu Gao3Mengxuan Du4Mengge Gao5Xingming Zhong6Xiangcai Wei7Department of Reproductive Immunity, Guangdong Women and Children Hospital, Guangzhou Medical University, Guangzhou, ChinaDepartment of Medical Ultrasonics, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaDepartment of Reproductive Immunity, Guangdong Women and Children Hospital, Guangzhou Medical University, Guangzhou, ChinaDepartment of Epidemiology, School of Medicine, Jinan University, Guangzhou, ChinaDepartment of Epidemiology, School of Medicine, Jinan University, Guangzhou, ChinaDepartment of Epidemiology, School of Medicine, Jinan University, Guangzhou, ChinaDepartment of Reproductive Immunity, Family Planning Research Institute of Guangdong Province, Guangzhou, ChinaDepartment of Reproductive Immunity, Guangdong Women and Children Hospital, Guangzhou Medical University, Guangzhou, ChinaPurposeThis study aimed to investigate the profiles of messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) in peripheral blood samples collected from polycystic ovary syndrome (PCOS) patients. In addition, an in-depth bioinformatics analysis regarding the lncRNA-mRNA co-expression network was performed.MethodsHigh-throughput sequencing was used to measure the profiles of mRNAs and lncRNAs expressed in the peripheral blood samples isolated from six patients (three patients with PCOS and three normal women). In addition, five differentially expressed lncRNAs were chosen to validate the results of high-throughput sequencing by quantitative RT-PCR (qRT-PCR). Furthermore, a lncRNA-mRNA co-expression network was constructed using the Cytoscape software.ResultsA total of 14,276 differentially expressed mRNAs and 4,048 differentially expressed lncRNAs were obtained from the RNA-seq analysis of PCOS patients and healthy controls (adjusted q-value < 0.05, Fold change >2.0).The qRT-PCR results were consistent with the data obtained through high-throughput sequencing. Gene ontology (GO) and KEGG pathway analyses showed that the differentially expressed mRNAs were enriched in the chemokine signaling pathway. In addition, the analysis of the lncRNA-mRNA co-expression network of the chemokine signaling pathway showed the involvement of 6 mRNAs and 42 lncRNAs.ConclusionClusters of mRNAs and lncRNAs were aberrantly expressed in the peripheral blood of PCOS patients compared with the controls. In addition, several pairs of lncRNA-mRNAs in the chemokine signaling pathway may be related to PCOS genetically.https://www.frontiersin.org/articles/10.3389/fimmu.2021.669819/fullpolycystic ovary syndromelong noncoding RNAmessenger RNAperipheral bloodgene ontologypathway analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiuhong Sun Xiuhong Sun Yishan Liu Xinyu Gao Mengxuan Du Mengge Gao Xingming Zhong Xiangcai Wei |
spellingShingle |
Xiuhong Sun Xiuhong Sun Yishan Liu Xinyu Gao Mengxuan Du Mengge Gao Xingming Zhong Xiangcai Wei Analysis of LncRNA-mRNA Co-Expression Profiles in Patients With Polycystic Ovary Syndrome: A Pilot Study Frontiers in Immunology polycystic ovary syndrome long noncoding RNA messenger RNA peripheral blood gene ontology pathway analysis |
author_facet |
Xiuhong Sun Xiuhong Sun Yishan Liu Xinyu Gao Mengxuan Du Mengge Gao Xingming Zhong Xiangcai Wei |
author_sort |
Xiuhong Sun |
title |
Analysis of LncRNA-mRNA Co-Expression Profiles in Patients With Polycystic Ovary Syndrome: A Pilot Study |
title_short |
Analysis of LncRNA-mRNA Co-Expression Profiles in Patients With Polycystic Ovary Syndrome: A Pilot Study |
title_full |
Analysis of LncRNA-mRNA Co-Expression Profiles in Patients With Polycystic Ovary Syndrome: A Pilot Study |
title_fullStr |
Analysis of LncRNA-mRNA Co-Expression Profiles in Patients With Polycystic Ovary Syndrome: A Pilot Study |
title_full_unstemmed |
Analysis of LncRNA-mRNA Co-Expression Profiles in Patients With Polycystic Ovary Syndrome: A Pilot Study |
title_sort |
analysis of lncrna-mrna co-expression profiles in patients with polycystic ovary syndrome: a pilot study |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2021-04-01 |
description |
PurposeThis study aimed to investigate the profiles of messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) in peripheral blood samples collected from polycystic ovary syndrome (PCOS) patients. In addition, an in-depth bioinformatics analysis regarding the lncRNA-mRNA co-expression network was performed.MethodsHigh-throughput sequencing was used to measure the profiles of mRNAs and lncRNAs expressed in the peripheral blood samples isolated from six patients (three patients with PCOS and three normal women). In addition, five differentially expressed lncRNAs were chosen to validate the results of high-throughput sequencing by quantitative RT-PCR (qRT-PCR). Furthermore, a lncRNA-mRNA co-expression network was constructed using the Cytoscape software.ResultsA total of 14,276 differentially expressed mRNAs and 4,048 differentially expressed lncRNAs were obtained from the RNA-seq analysis of PCOS patients and healthy controls (adjusted q-value < 0.05, Fold change >2.0).The qRT-PCR results were consistent with the data obtained through high-throughput sequencing. Gene ontology (GO) and KEGG pathway analyses showed that the differentially expressed mRNAs were enriched in the chemokine signaling pathway. In addition, the analysis of the lncRNA-mRNA co-expression network of the chemokine signaling pathway showed the involvement of 6 mRNAs and 42 lncRNAs.ConclusionClusters of mRNAs and lncRNAs were aberrantly expressed in the peripheral blood of PCOS patients compared with the controls. In addition, several pairs of lncRNA-mRNAs in the chemokine signaling pathway may be related to PCOS genetically. |
topic |
polycystic ovary syndrome long noncoding RNA messenger RNA peripheral blood gene ontology pathway analysis |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.669819/full |
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