In Vivo 3T Magnetic Resonance Imaging Using a Biologically Specific Contrast Agent for Prostate Cancer: A Nude Mouse Model
We characterized in vivo a functional superparamagnetic iron-oxide magnetic resonance contrast agent that shortens the T2 relaxation time in magnetic resonance imaging (MRI) of prostate cancer xenografts. The agent was developed by conjugating Molday ION™ carboxyl-6 (MIC6), with a deimmunized mouse...
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doaj-1ba043ea0c8a43ebb7457a264b2cfb022020-11-24T23:14:28ZengHindawi LimitedJournal of Nanotechnology1687-95031687-95112017-01-01201710.1155/2017/84246868424686In Vivo 3T Magnetic Resonance Imaging Using a Biologically Specific Contrast Agent for Prostate Cancer: A Nude Mouse ModelChristopher Brian Abraham0Prashant Jani1Roxanne Turuba2Michael Campbell3Ingeborg Zehbe4Laura Curiel5Department of Electrical Engineering, Lakehead University, Thunder Bay, ON, CanadaDepartment of Pathology, Thunder Bay Regional Health Sciences Centre, Thunder Bay, ON, CanadaDepartment of Biology, Lakehead University, Thunder Bay, ON, CanadaDepartment of Chemistry, Lakehead University, Thunder Bay, ON, CanadaDepartment of Biology, Lakehead University, Thunder Bay, ON, CanadaDepartment of Electrical Engineering, Lakehead University, Thunder Bay, ON, CanadaWe characterized in vivo a functional superparamagnetic iron-oxide magnetic resonance contrast agent that shortens the T2 relaxation time in magnetic resonance imaging (MRI) of prostate cancer xenografts. The agent was developed by conjugating Molday ION™ carboxyl-6 (MIC6), with a deimmunized mouse monoclonal antibody (muJ591) targeting prostate-specific membrane antigen (PSMA). This functional contrast agent could be used as a noninvasive method to detect prostate cancer cells that are PSMA positive and more readily differentiate them from surrounding tissues for treatment. The functional contrast agent was injected intravenously into mice and its effect was compared to both MIC6 (without conjugated antibody) and phosphate-buffered saline (PBS) injection controls. MR imaging was performed on a clinical 3T MRI scanner using a multiecho spin echo (MESE) sequence to obtain T2 relaxation time values. Inductively coupled plasma atomic emission spectroscopy was used to confirm an increase in elemental iron in injected mice tumours relative to controls. Histological examination of H&E stained tissues showed normal morphology of the tissues collected.http://dx.doi.org/10.1155/2017/8424686 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christopher Brian Abraham Prashant Jani Roxanne Turuba Michael Campbell Ingeborg Zehbe Laura Curiel |
spellingShingle |
Christopher Brian Abraham Prashant Jani Roxanne Turuba Michael Campbell Ingeborg Zehbe Laura Curiel In Vivo 3T Magnetic Resonance Imaging Using a Biologically Specific Contrast Agent for Prostate Cancer: A Nude Mouse Model Journal of Nanotechnology |
author_facet |
Christopher Brian Abraham Prashant Jani Roxanne Turuba Michael Campbell Ingeborg Zehbe Laura Curiel |
author_sort |
Christopher Brian Abraham |
title |
In Vivo 3T Magnetic Resonance Imaging Using a Biologically Specific Contrast Agent for Prostate Cancer: A Nude Mouse Model |
title_short |
In Vivo 3T Magnetic Resonance Imaging Using a Biologically Specific Contrast Agent for Prostate Cancer: A Nude Mouse Model |
title_full |
In Vivo 3T Magnetic Resonance Imaging Using a Biologically Specific Contrast Agent for Prostate Cancer: A Nude Mouse Model |
title_fullStr |
In Vivo 3T Magnetic Resonance Imaging Using a Biologically Specific Contrast Agent for Prostate Cancer: A Nude Mouse Model |
title_full_unstemmed |
In Vivo 3T Magnetic Resonance Imaging Using a Biologically Specific Contrast Agent for Prostate Cancer: A Nude Mouse Model |
title_sort |
in vivo 3t magnetic resonance imaging using a biologically specific contrast agent for prostate cancer: a nude mouse model |
publisher |
Hindawi Limited |
series |
Journal of Nanotechnology |
issn |
1687-9503 1687-9511 |
publishDate |
2017-01-01 |
description |
We characterized in vivo a functional superparamagnetic iron-oxide magnetic resonance contrast agent that shortens the T2 relaxation time in magnetic resonance imaging (MRI) of prostate cancer xenografts. The agent was developed by conjugating Molday ION™ carboxyl-6 (MIC6), with a deimmunized mouse monoclonal antibody (muJ591) targeting prostate-specific membrane antigen (PSMA). This functional contrast agent could be used as a noninvasive method to detect prostate cancer cells that are PSMA positive and more readily differentiate them from surrounding tissues for treatment. The functional contrast agent was injected intravenously into mice and its effect was compared to both MIC6 (without conjugated antibody) and phosphate-buffered saline (PBS) injection controls. MR imaging was performed on a clinical 3T MRI scanner using a multiecho spin echo (MESE) sequence to obtain T2 relaxation time values. Inductively coupled plasma atomic emission spectroscopy was used to confirm an increase in elemental iron in injected mice tumours relative to controls. Histological examination of H&E stained tissues showed normal morphology of the tissues collected. |
url |
http://dx.doi.org/10.1155/2017/8424686 |
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