New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt
Icariin has been demonstrated to stimulate mesenchymal stem cell (MSC) osteogensis and activate several signals, such as PI3K/Akt, but how the osteogenesis was sequentially mediated is unclear. Runx2 is one of the osteogenic regulators in MSC and is regulated by the TAZ gene. The purpose of this stu...
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doaj-1ba7eed43b8f40dc81fda07c0965ac1b2021-09-05T20:42:21ZengDe GruyterOpen Life Sciences2391-54122017-09-0112122823610.1515/biol-2017-0027biol-2017-0027New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/AktZhang Guoying0Cheng Xiaofei1Zhou Gongshe2Xue Huimin3Shao Shan4Wang Zheng5Department of Orthopedics, The General Hospital of Chinese People’s Liberation Army, 28 Fuxing Road, 100853, Beijing, ChinaShanghai Key Laboratory of Orthopaedic Implants, Departmemt of Orthopaedic Surgery, Ninth People’s Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, ChinaDepartment of Orthopedics, The Center Hospital of Zhoukou, Henan Province, ChinaThe Third People’s Hospital of Jinan. 1 North Industrial Road, Wangsheren North Street, Jinan 250132, Shandong Province, ChinaThe Third People’s Hospital of Jinan. 1 North Industrial Road, Wangsheren North Street, Jinan 250132, Shandong Province, ChinaDepartment of Orthopedics, The General Hospital of Chinese People’s Liberation Army, 28 Fuxing Road, 100853, Beijing, ChinaIcariin has been demonstrated to stimulate mesenchymal stem cell (MSC) osteogensis and activate several signals, such as PI3K/Akt, but how the osteogenesis was sequentially mediated is unclear. Runx2 is one of the osteogenic regulators in MSC and is regulated by the TAZ gene. The purpose of this study was to investigate whether icariin-activated PI3K/Akt crosstalked with the TAZ-Runx2 pathway to regulate MSC osteogenesis. Adipose-derived MSCs were treated with icariin alone, together with TAZ silencing or PI3K/Akt inhibitor. Normal MSCs were used as a control. The activation of PI3K/Akt, expression of TAZ and downstream expression of Runx2 were analyzed. Induction of MSC osteogenesis under different treatments was detected. The results demonstrated that icariin treatment significantly activated PI3K/Akt and TAZ expression, as well as the downstream Runx2 expression. When activation of PI3K/Akt by icariin was inhibited by LY294002, upregulated TAZ expression was reversed, as well as the downstream expression of Runx2. Consequently, with the osteogenic counteracting effects of icariin on MSCs, inhibition of TAZ upregulation by siRNA did not significantly influence PI3K/ Akt activation in icariin-treated MSCs, but icariin-induced upregulation of Runx2 and osteogenic differentiation in MSCs was counteracted. It could be concluded from these findings that icariin treatment activated PI3K/Akt and further mediated the transcriptional activation of the TAZ/Runx2 pathway to induce osteogenic differentiation of MSCs.https://doi.org/10.1515/biol-2017-0027icariinpi3k/akttaz/runxmscsosteogenic differentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhang Guoying Cheng Xiaofei Zhou Gongshe Xue Huimin Shao Shan Wang Zheng |
spellingShingle |
Zhang Guoying Cheng Xiaofei Zhou Gongshe Xue Huimin Shao Shan Wang Zheng New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt Open Life Sciences icariin pi3k/akt taz/runx mscs osteogenic differentiation |
author_facet |
Zhang Guoying Cheng Xiaofei Zhou Gongshe Xue Huimin Shao Shan Wang Zheng |
author_sort |
Zhang Guoying |
title |
New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt |
title_short |
New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt |
title_full |
New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt |
title_fullStr |
New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt |
title_full_unstemmed |
New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt |
title_sort |
new pathway of icariin-induced msc osteogenesis: transcriptional activation of taz/runx2 by pi3k/akt |
publisher |
De Gruyter |
series |
Open Life Sciences |
issn |
2391-5412 |
publishDate |
2017-09-01 |
description |
Icariin has been demonstrated to stimulate mesenchymal stem cell (MSC) osteogensis and activate several signals, such as PI3K/Akt, but how the osteogenesis was sequentially mediated is unclear. Runx2 is one of the osteogenic regulators in MSC and is regulated by the TAZ gene. The purpose of this study was to investigate whether icariin-activated PI3K/Akt crosstalked with the TAZ-Runx2 pathway to regulate MSC osteogenesis. Adipose-derived MSCs were treated with icariin alone, together with TAZ silencing or PI3K/Akt inhibitor. Normal MSCs were used as a control. The activation of PI3K/Akt, expression of TAZ and downstream expression of Runx2 were analyzed. Induction of MSC osteogenesis under different treatments was detected. The results demonstrated that icariin treatment significantly activated PI3K/Akt and TAZ expression, as well as the downstream Runx2 expression. When activation of PI3K/Akt by icariin was inhibited by LY294002, upregulated TAZ expression was reversed, as well as the downstream expression of Runx2. Consequently, with the osteogenic counteracting effects of icariin on MSCs, inhibition of TAZ upregulation by siRNA did not significantly influence PI3K/ Akt activation in icariin-treated MSCs, but icariin-induced upregulation of Runx2 and osteogenic differentiation in MSCs was counteracted. It could be concluded from these findings that icariin treatment activated PI3K/Akt and further mediated the transcriptional activation of the TAZ/Runx2 pathway to induce osteogenic differentiation of MSCs. |
topic |
icariin pi3k/akt taz/runx mscs osteogenic differentiation |
url |
https://doi.org/10.1515/biol-2017-0027 |
work_keys_str_mv |
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