Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine.
Enteroendocrine cells are solitary epithelial cells scattered throughout the gastrointestinal tract and produce various types of hormones, constituting one of the largest endocrine systems in the body. The study of these rare epithelial cells has been hampered by the difficulty in isolating them bec...
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doaj-1bb7965db26c4a6c93dc2d81d6ea24132020-11-25T01:26:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9063810.1371/journal.pone.0090638Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine.Takahiro NagatakeHarumi FujitaNagahiro MinatoYoko HamazakiEnteroendocrine cells are solitary epithelial cells scattered throughout the gastrointestinal tract and produce various types of hormones, constituting one of the largest endocrine systems in the body. The study of these rare epithelial cells has been hampered by the difficulty in isolating them because of the lack of specific cell surface markers. Here, we report that enteroendocrine cells selectively express a tight junction membrane protein, claudin-4 (Cld4), and are efficiently isolated with the use of an antibody specific for the Cld4 extracellular domain and flow cytometry. Sorted Cld4+ epithelial cells in the small intestine exclusively expressed a chromogranin A gene (Chga) and other enteroendocrine cell-related genes (Ffar1, Ffar4, Gpr119), and the population was divided into two subpopulations based on the activity of binding to Ulex europaeus agglutinin-1 (UEA-1). A Cld4+UEA-1- cell population almost exclusively expressed glucose-dependent insulinotropic polypeptide gene (Gip), thus representing K cells, whereas a Cld4+UEA-1+ cell population expressed other gut hormone genes, including glucagon-like peptide 1 (Gcg), pancreatic polypeptide-like peptide with N-terminal tyrosine amide (Pyy), cholecystokinin (Cck), secretin (Sct), and tryptophan hydroxylase 1 (Tph1). In addition, we found that orally administered luminal antigens were taken up by the solitary Cld4+ cells in the small intestinal villi, raising the possibility that enteroendocrine cells might also play a role in initiation of mucosal immunity. Our results provide a useful tool for the cellular and functional characterization of enteroendocrine cells.http://europepmc.org/articles/PMC3948345?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takahiro Nagatake Harumi Fujita Nagahiro Minato Yoko Hamazaki |
spellingShingle |
Takahiro Nagatake Harumi Fujita Nagahiro Minato Yoko Hamazaki Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine. PLoS ONE |
author_facet |
Takahiro Nagatake Harumi Fujita Nagahiro Minato Yoko Hamazaki |
author_sort |
Takahiro Nagatake |
title |
Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine. |
title_short |
Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine. |
title_full |
Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine. |
title_fullStr |
Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine. |
title_full_unstemmed |
Enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine. |
title_sort |
enteroendocrine cells are specifically marked by cell surface expression of claudin-4 in mouse small intestine. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Enteroendocrine cells are solitary epithelial cells scattered throughout the gastrointestinal tract and produce various types of hormones, constituting one of the largest endocrine systems in the body. The study of these rare epithelial cells has been hampered by the difficulty in isolating them because of the lack of specific cell surface markers. Here, we report that enteroendocrine cells selectively express a tight junction membrane protein, claudin-4 (Cld4), and are efficiently isolated with the use of an antibody specific for the Cld4 extracellular domain and flow cytometry. Sorted Cld4+ epithelial cells in the small intestine exclusively expressed a chromogranin A gene (Chga) and other enteroendocrine cell-related genes (Ffar1, Ffar4, Gpr119), and the population was divided into two subpopulations based on the activity of binding to Ulex europaeus agglutinin-1 (UEA-1). A Cld4+UEA-1- cell population almost exclusively expressed glucose-dependent insulinotropic polypeptide gene (Gip), thus representing K cells, whereas a Cld4+UEA-1+ cell population expressed other gut hormone genes, including glucagon-like peptide 1 (Gcg), pancreatic polypeptide-like peptide with N-terminal tyrosine amide (Pyy), cholecystokinin (Cck), secretin (Sct), and tryptophan hydroxylase 1 (Tph1). In addition, we found that orally administered luminal antigens were taken up by the solitary Cld4+ cells in the small intestinal villi, raising the possibility that enteroendocrine cells might also play a role in initiation of mucosal immunity. Our results provide a useful tool for the cellular and functional characterization of enteroendocrine cells. |
url |
http://europepmc.org/articles/PMC3948345?pdf=render |
work_keys_str_mv |
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