Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis
Mastitis is an inflammatory disorder caused by microorganisms. Currently antibiotics have been mainstay of mastitis therapy.However, their use is associated with cost issue and human health concern. Some herbs exert beneficial effects on bacterial pathogens through immunomodulation by influencing c...
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doaj-1bc073a11d804deaa0f9c6ba124bee7b2020-11-24T22:48:54ZengScience Planet Inc.Canadian Journal of Biotechnology2560-83042017-10-011Special Issue15115110.24870/cjb.2017-a137Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitisMukesh Kher0Vaibhav Bhatt1Navin Sheth2Anju Kunjadia3Dev Nauriyal4Chaitanya Joshi5Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, INDIADepartment of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, INDIADepartment of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, INDIACenter for interdisciplinary studies in science and technology (CISST), Sardar Patel University, Vallabh Vidyanagar, Gujarat, INDIADepartments of Veterinary Medicine, Veterinary Science College, AAU, Anand, Gujarat, INDIADepartments of Animal Biotechnology, Veterinary Science College, AAU, Anand, Gujarat, INDIAMastitis is an inflammatory disorder caused by microorganisms. Currently antibiotics have been mainstay of mastitis therapy.However, their use is associated with cost issue and human health concern. Some herbs exert beneficial effects on bacterial pathogens through immunomodulation by influencing cytokine production. To assess the effect of herbs on cytokine profile, total bacterial load and somatic cell count in two breeds of cattle harboring subclinical mastitis. The response to treatment was evaluated by enumerating somatic cell count, total bacterial load and studying the expression of different cytokines (IL-6, IL-8, IL-12, IFN-Ɣ and TNF-α).The expression profiles were carried out using real time PCR, by collecting milk on days 0 as well as 5 and 21 post last treatment and data were analyzed using Statistical analysis system software. Pre and post treatment SCC in mastitic quarters statistically did not differ significantly, however, total bacterial load declined significantly from day 0 onwards in both the breeds. Highly significant differences (P < 0.01) were observed in all the cytokines on day 0, 5, and 21 post last treatments in both the breeds. The comparison between crossbred and Gir cattle revealed a significant difference in expression of IIL-6 and TNF-α. However, other cytokines exhibited a similar pattern of expression in both breeds, which was non-significant. The topical herbal drug exhibited antibacterial and immunomodulatory activities and thus the work supports its use as an alternative to antibiotics against subclinical udder infection in bovines.https://www.canadianjbiotech.com/CAN_J_BIOTECH/Archives/v1/Special Issue/cjb.2017-a137.pdf |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mukesh Kher Vaibhav Bhatt Navin Sheth Anju Kunjadia Dev Nauriyal Chaitanya Joshi |
spellingShingle |
Mukesh Kher Vaibhav Bhatt Navin Sheth Anju Kunjadia Dev Nauriyal Chaitanya Joshi Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis Canadian Journal of Biotechnology |
author_facet |
Mukesh Kher Vaibhav Bhatt Navin Sheth Anju Kunjadia Dev Nauriyal Chaitanya Joshi |
author_sort |
Mukesh Kher |
title |
Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis |
title_short |
Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis |
title_full |
Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis |
title_fullStr |
Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis |
title_full_unstemmed |
Evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis |
title_sort |
evaluation of a topical herbal drug for its in-vivo immunological effect on cytokines production and antibacterial activity in bovine subclinical mastitis |
publisher |
Science Planet Inc. |
series |
Canadian Journal of Biotechnology |
issn |
2560-8304 |
publishDate |
2017-10-01 |
description |
Mastitis is an inflammatory disorder caused by microorganisms. Currently antibiotics have been mainstay of mastitis therapy.However, their use is associated with cost issue and human health concern. Some herbs exert beneficial effects on bacterial
pathogens through immunomodulation by influencing cytokine production. To assess the effect of herbs on cytokine profile, total
bacterial load and somatic cell count in two breeds of cattle harboring subclinical mastitis. The response to treatment was
evaluated by enumerating somatic cell count, total bacterial load and studying the expression of different cytokines (IL-6, IL-8,
IL-12, IFN-Ɣ and TNF-α).The expression profiles were carried out using real time PCR, by collecting milk on days 0 as well as 5
and 21 post last treatment and data were analyzed using Statistical analysis system software. Pre and post treatment SCC in
mastitic quarters statistically did not differ significantly, however, total bacterial load declined significantly from day 0 onwards
in both the breeds. Highly significant differences (P < 0.01) were observed in all the cytokines on day 0, 5, and 21 post last
treatments in both the breeds. The comparison between crossbred and Gir cattle revealed a significant difference in expression of
IIL-6 and TNF-α. However, other cytokines exhibited a similar pattern of expression in both breeds, which was non-significant.
The topical herbal drug exhibited antibacterial and immunomodulatory activities and thus the work supports its use as an
alternative to antibiotics against subclinical udder infection in bovines. |
url |
https://www.canadianjbiotech.com/CAN_J_BIOTECH/Archives/v1/Special Issue/cjb.2017-a137.pdf |
work_keys_str_mv |
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