Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime
The biomass and quality of Pseudostellariae heterophylla suffers a significant decline under monoculture. Since rhizosphere microbiome plays crucial roles in soil health, deep pyrosequencing combined with qPCR was applied to characterize the composition and structure of soil bacterial community unde...
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Frontiers Media S.A.
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doaj-1bc7ae58beff48b7a07f6ab4cc0d59c82020-11-24T23:16:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01788218466Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture RegimeLinkun Wu0Linkun Wu1Jun Chen2Jun Chen3Hongmiao Wu4Hongmiao Wu5Xianjin Qin6Xianjin Qin7Juanying Wang8Juanying Wang9Yanhong Wu10Yanhong Wu11Muhammad Umar Khan12Muhammad Umar Khan13Sheng Lin14Sheng Lin15Zhigang Xiao16Zhigang Xiao17Xiaomian Luo18Xiaomian Luo19Zhongyi Zhang20Zhongyi Zhang21Wenxiong Lin22Wenxiong Lin23Fujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityFujian Agriculture and Forestry UniversityThe biomass and quality of Pseudostellariae heterophylla suffers a significant decline under monoculture. Since rhizosphere microbiome plays crucial roles in soil health, deep pyrosequencing combined with qPCR was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. The results showed compared with the first-year planted (FP), second-year monoculture of P. heterophylla (SP) led to a significant decline in yield and resulted in a significant increase in Fusarium oxysporum but a decline in Burkholderia spp. Bio-organic fertilizer (MT) formulated by combining antagonistic bacteria with organic matter could significantly promote the yield by regulating rhizosphere bacterial community. However, organic fertilizer (MO) without antagonistic bacteria could not suppress Fusarium wilt. Multivariate statistics analysis showed a distinct separation between the healthy samples (FP and MT) and the unhealthy samples (SP and MO), suggesting a strong relationship between soil microbial community and plant performance. Furthermore, we found the application of bio-organic fertilizer MT could significantly increase the bacterial community diversity and restructure microbial community with relatively fewer pathogenic F. oxysporum and more beneficial Burkholderia spp. In conclusion, the application of novel bio-organic fertilizer could effectively suppress Fusarium wilt by enriching the antagonistic bacteria and enhancing the bacterial diversity.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01788/fullmicrobial communityPseudostellaria heterophyllaBio-organic fertilizerReplant diseasedeep pyrosequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Linkun Wu Linkun Wu Jun Chen Jun Chen Hongmiao Wu Hongmiao Wu Xianjin Qin Xianjin Qin Juanying Wang Juanying Wang Yanhong Wu Yanhong Wu Muhammad Umar Khan Muhammad Umar Khan Sheng Lin Sheng Lin Zhigang Xiao Zhigang Xiao Xiaomian Luo Xiaomian Luo Zhongyi Zhang Zhongyi Zhang Wenxiong Lin Wenxiong Lin |
spellingShingle |
Linkun Wu Linkun Wu Jun Chen Jun Chen Hongmiao Wu Hongmiao Wu Xianjin Qin Xianjin Qin Juanying Wang Juanying Wang Yanhong Wu Yanhong Wu Muhammad Umar Khan Muhammad Umar Khan Sheng Lin Sheng Lin Zhigang Xiao Zhigang Xiao Xiaomian Luo Xiaomian Luo Zhongyi Zhang Zhongyi Zhang Wenxiong Lin Wenxiong Lin Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime Frontiers in Microbiology microbial community Pseudostellaria heterophylla Bio-organic fertilizer Replant disease deep pyrosequencing |
author_facet |
Linkun Wu Linkun Wu Jun Chen Jun Chen Hongmiao Wu Hongmiao Wu Xianjin Qin Xianjin Qin Juanying Wang Juanying Wang Yanhong Wu Yanhong Wu Muhammad Umar Khan Muhammad Umar Khan Sheng Lin Sheng Lin Zhigang Xiao Zhigang Xiao Xiaomian Luo Xiaomian Luo Zhongyi Zhang Zhongyi Zhang Wenxiong Lin Wenxiong Lin |
author_sort |
Linkun Wu |
title |
Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime |
title_short |
Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime |
title_full |
Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime |
title_fullStr |
Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime |
title_full_unstemmed |
Insights into the Regulation of Rhizosphere Bacterial Communities by Application of Bio-organic Fertilizer in Pseudostellaria heterophylla Monoculture Regime |
title_sort |
insights into the regulation of rhizosphere bacterial communities by application of bio-organic fertilizer in pseudostellaria heterophylla monoculture regime |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-11-01 |
description |
The biomass and quality of Pseudostellariae heterophylla suffers a significant decline under monoculture. Since rhizosphere microbiome plays crucial roles in soil health, deep pyrosequencing combined with qPCR was applied to characterize the composition and structure of soil bacterial community under monoculture and different amendments. The results showed compared with the first-year planted (FP), second-year monoculture of P. heterophylla (SP) led to a significant decline in yield and resulted in a significant increase in Fusarium oxysporum but a decline in Burkholderia spp. Bio-organic fertilizer (MT) formulated by combining antagonistic bacteria with organic matter could significantly promote the yield by regulating rhizosphere bacterial community. However, organic fertilizer (MO) without antagonistic bacteria could not suppress Fusarium wilt. Multivariate statistics analysis showed a distinct separation between the healthy samples (FP and MT) and the unhealthy samples (SP and MO), suggesting a strong relationship between soil microbial community and plant performance. Furthermore, we found the application of bio-organic fertilizer MT could significantly increase the bacterial community diversity and restructure microbial community with relatively fewer pathogenic F. oxysporum and more beneficial Burkholderia spp. In conclusion, the application of novel bio-organic fertilizer could effectively suppress Fusarium wilt by enriching the antagonistic bacteria and enhancing the bacterial diversity. |
topic |
microbial community Pseudostellaria heterophylla Bio-organic fertilizer Replant disease deep pyrosequencing |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01788/full |
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