Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers
Tubular vascular grafts 1.1 mm in diameter based on poly(L-lactide) microfibers were obtained by electrospinning. X-ray diffraction and scanning electron microscopy data demonstrated that the samples treated at T=70°C for 1 h in the fixed state on a cylindrical mandrel possessed dense fibrous struct...
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Series: | International Journal of Biomaterials |
Online Access: | http://dx.doi.org/10.1155/2017/9034186 |
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doaj-1bcad943fbb1440a811006e620bb33f62020-11-25T01:07:31ZengHindawi LimitedInternational Journal of Biomaterials1687-87871687-87952017-01-01201710.1155/2017/90341869034186Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide MicrofibersP. V. Popryadukhin0G. I. Popov1G. Yu. Yukina2I. P. Dobrovolskaya3E. M. Ivan’kova4V. N. Vavilov5V. E. Yudin6Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy Pr. 31, Saint-Petersburg 199004, RussiaPavlov First Saint-Petersburg State Medical University, Leo Tolstoy Str. 6-8, Saint-Petersburg 197022, RussiaPavlov First Saint-Petersburg State Medical University, Leo Tolstoy Str. 6-8, Saint-Petersburg 197022, RussiaInstitute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy Pr. 31, Saint-Petersburg 199004, RussiaInstitute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy Pr. 31, Saint-Petersburg 199004, RussiaPavlov First Saint-Petersburg State Medical University, Leo Tolstoy Str. 6-8, Saint-Petersburg 197022, RussiaInstitute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy Pr. 31, Saint-Petersburg 199004, RussiaTubular vascular grafts 1.1 mm in diameter based on poly(L-lactide) microfibers were obtained by electrospinning. X-ray diffraction and scanning electron microscopy data demonstrated that the samples treated at T=70°C for 1 h in the fixed state on a cylindrical mandrel possessed dense fibrous structure; their degree of crystallinity was approximately 44%. Strength and deformation stability of these samples were higher than those of the native blood vessels; thus, it was possible to use them in tissue engineering as bioresorbable vascular grafts. The experiments on including implantation into rat abdominal aorta demonstrated that the obtained vascular grafts did not cause pathological reactions in the rats; in four weeks, inner side of the grafts became completely covered with endothelial cells, and fibroblasts grew throughout the wall. After exposure for 12 weeks, resorption of PLLA fibers started, and this process was completed in 64 weeks. Resorbed synthetic fibers were replaced by collagen and fibroblasts. At that time, the blood vessel was formed; its neointima and neoadventitia were close to those of the native vessel in structure and composition.http://dx.doi.org/10.1155/2017/9034186 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
P. V. Popryadukhin G. I. Popov G. Yu. Yukina I. P. Dobrovolskaya E. M. Ivan’kova V. N. Vavilov V. E. Yudin |
spellingShingle |
P. V. Popryadukhin G. I. Popov G. Yu. Yukina I. P. Dobrovolskaya E. M. Ivan’kova V. N. Vavilov V. E. Yudin Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers International Journal of Biomaterials |
author_facet |
P. V. Popryadukhin G. I. Popov G. Yu. Yukina I. P. Dobrovolskaya E. M. Ivan’kova V. N. Vavilov V. E. Yudin |
author_sort |
P. V. Popryadukhin |
title |
Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers |
title_short |
Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers |
title_full |
Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers |
title_fullStr |
Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers |
title_full_unstemmed |
Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers |
title_sort |
tissue-engineered vascular graft of small diameter based on electrospun polylactide microfibers |
publisher |
Hindawi Limited |
series |
International Journal of Biomaterials |
issn |
1687-8787 1687-8795 |
publishDate |
2017-01-01 |
description |
Tubular vascular grafts 1.1 mm in diameter based on poly(L-lactide) microfibers were obtained by electrospinning. X-ray diffraction and scanning electron microscopy data demonstrated that the samples treated at T=70°C for 1 h in the fixed state on a cylindrical mandrel possessed dense fibrous structure; their degree of crystallinity was approximately 44%. Strength and deformation stability of these samples were higher than those of the native blood vessels; thus, it was possible to use them in tissue engineering as bioresorbable vascular grafts. The experiments on including implantation into rat abdominal aorta demonstrated that the obtained vascular grafts did not cause pathological reactions in the rats; in four weeks, inner side of the grafts became completely covered with endothelial cells, and fibroblasts grew throughout the wall. After exposure for 12 weeks, resorption of PLLA fibers started, and this process was completed in 64 weeks. Resorbed synthetic fibers were replaced by collagen and fibroblasts. At that time, the blood vessel was formed; its neointima and neoadventitia were close to those of the native vessel in structure and composition. |
url |
http://dx.doi.org/10.1155/2017/9034186 |
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