Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study
Introduction Respiratory tract lining fluid of small airways mainly consists of surfactant that can be investigated by collection of the particles of exhaled aerosol (PExA) method. This offers an exciting prospect to monitor small airway pathology, including subjects with asthma and smokers.Aim To e...
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doaj-1bd4a6d8261040e5ba3ceb497c284f8d2021-08-10T11:30:04ZengBMJ Publishing GroupBMJ Open Respiratory Research2052-44392021-08-018110.1136/bmjresp-2020-000804Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort studyAnna-Carin Olin0Laith Hussain-Alkhateeb1Björn Bake2Össur Emilsson3Ekaterina Mirgorodskaya4Public Health and Community Medicine, University of Gothenburg Sahlgrenska Academy, Goteborg, SwedenPublic Health and Community Medicine, University of Gothenburg Sahlgrenska Academy, Goteborg, SwedenDepartment of Respiratory Medicine and Allergology, Insitute of Medicine, Gothenburg, SwedenDepartment of Medical Sciences, Respiratory, allergy and sleep research, Uppsala University, Uppsala, SwedenProteomics Core Facility, Sahlgrnska Acadey, University of Gothenburg, Gothenburg, SwedenIntroduction Respiratory tract lining fluid of small airways mainly consists of surfactant that can be investigated by collection of the particles of exhaled aerosol (PExA) method. This offers an exciting prospect to monitor small airway pathology, including subjects with asthma and smokers.Aim To explore the influence of anthropometric factors and gender on phospholipids, surfactant protein A (SP-A) and albumin of the lining fluid of small airwaysand to examine the association with asthma and smoking. Furthermore, to examine if the surfactant components can predict lung function in terms of spirometry variables.Method This study employs the population-based cohort of the European Community Respiratory Health Survey III, including participants from Gothenburg city, Sweden (n=200). The PExA method enabled quantitative description and analytical analysis of phospholipids, SP-A and albumin of the lining fluid of small airways.Results Age was a significant predictor of the phospholipids. The components PC14:0/16:0, PC16:0/18:2 (PC, phosphatidylcholine) and SP-A were higher among subjects with asthma, whereas albumin was lower. Among smokers, there were higher levels particularly of di-palmitoyl-di-phosphatidyl-choline compared with non-smokers. Most phospholipids significantly predicted the spirometry variables.Conclusion This non-invasive PExA method appears to have great potential to explore the role of lipids and proteins of surfactant in respiratory disease.https://bmjopenrespres.bmj.com/content/8/1/e000804.full |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna-Carin Olin Laith Hussain-Alkhateeb Björn Bake Össur Emilsson Ekaterina Mirgorodskaya |
spellingShingle |
Anna-Carin Olin Laith Hussain-Alkhateeb Björn Bake Össur Emilsson Ekaterina Mirgorodskaya Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study BMJ Open Respiratory Research |
author_facet |
Anna-Carin Olin Laith Hussain-Alkhateeb Björn Bake Össur Emilsson Ekaterina Mirgorodskaya |
author_sort |
Anna-Carin Olin |
title |
Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study |
title_short |
Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study |
title_full |
Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study |
title_fullStr |
Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study |
title_full_unstemmed |
Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study |
title_sort |
novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a european population-based cohort study |
publisher |
BMJ Publishing Group |
series |
BMJ Open Respiratory Research |
issn |
2052-4439 |
publishDate |
2021-08-01 |
description |
Introduction Respiratory tract lining fluid of small airways mainly consists of surfactant that can be investigated by collection of the particles of exhaled aerosol (PExA) method. This offers an exciting prospect to monitor small airway pathology, including subjects with asthma and smokers.Aim To explore the influence of anthropometric factors and gender on phospholipids, surfactant protein A (SP-A) and albumin of the lining fluid of small airwaysand to examine the association with asthma and smoking. Furthermore, to examine if the surfactant components can predict lung function in terms of spirometry variables.Method This study employs the population-based cohort of the European Community Respiratory Health Survey III, including participants from Gothenburg city, Sweden (n=200). The PExA method enabled quantitative description and analytical analysis of phospholipids, SP-A and albumin of the lining fluid of small airways.Results Age was a significant predictor of the phospholipids. The components PC14:0/16:0, PC16:0/18:2 (PC, phosphatidylcholine) and SP-A were higher among subjects with asthma, whereas albumin was lower. Among smokers, there were higher levels particularly of di-palmitoyl-di-phosphatidyl-choline compared with non-smokers. Most phospholipids significantly predicted the spirometry variables.Conclusion This non-invasive PExA method appears to have great potential to explore the role of lipids and proteins of surfactant in respiratory disease. |
url |
https://bmjopenrespres.bmj.com/content/8/1/e000804.full |
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