Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells
We studied the mtDNA bottleneck in zebrafish to elucidate size, timing, and variation in germline and non-germline cells. Mature zebrafish oocytes contain, on average, 19.0 × 106 mtDNA molecules with high variation between oocytes. During embryogenesis, the mtDNA copy number decreases to ∼170 mtDNA...
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doaj-1bd81a4cf5ce496a83cbe54fef730c282020-11-24T22:01:24ZengElsevierCell Reports2211-12472016-07-0116362263010.1016/j.celrep.2016.06.023Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline CellsAuke B.C. Otten0Tom E.J. Theunissen1Josien G. Derhaag2Ellen H. Lambrichs3Iris B.W. Boesten4Marie Winandy5Aafke P.A. van Montfoort6Katsiaryna Tarbashevich7Erez Raz8Mike Gerards9Jo M. Vanoevelen10Bianca J.C. van den Bosch11Marc Muller12Hubert J.M. Smeets13Department of Genetics and Cell Biology, Clinical Genomics Unit, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsDepartment of Genetics and Cell Biology, Clinical Genomics Unit, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsDepartment of Obstetrics and Gynaecology, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsDepartment of Genetics and Cell Biology, Clinical Genomics Unit, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsDepartment of Genetics and Cell Biology, Clinical Genomics Unit, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsLaboratory of Organogenesis and Regeneration, GIGA-Research, Univérsité de Liège, 4000 Liège, BelgiumDepartment of Obstetrics and Gynaecology, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsInstitute for Cell Biology, Centre for Molecular Biology of Inflammation, Münster University, 48149 Münster, GermanyInstitute for Cell Biology, Centre for Molecular Biology of Inflammation, Münster University, 48149 Münster, GermanyMaastricht Centre for Systems Biology (MaCSBio), Maastricht University Medical Centre, 6200MD, the NetherlandsDepartment of Genetics and Cell Biology, Clinical Genomics Unit, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsDepartment of Genetics and Cell Biology, Clinical Genomics Unit, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsLaboratory of Organogenesis and Regeneration, GIGA-Research, Univérsité de Liège, 4000 Liège, BelgiumDepartment of Genetics and Cell Biology, Clinical Genomics Unit, School for Oncology and Developmental Biology (GROW), Maastricht University Medical Centre, 6200MD Maastricht, the NetherlandsWe studied the mtDNA bottleneck in zebrafish to elucidate size, timing, and variation in germline and non-germline cells. Mature zebrafish oocytes contain, on average, 19.0 × 106 mtDNA molecules with high variation between oocytes. During embryogenesis, the mtDNA copy number decreases to ∼170 mtDNA molecules per primordial germ cell (PGC), a number similar to that in mammals, and to ∼50 per non-PGC. These occur at the same developmental stage, implying considerable variation in mtDNA copy number in (non-)PGCs of the same female, dictated by variation in the mature oocyte. The presence of oocytes with low mtDNA numbers, if similar in humans, could explain how (de novo) mutations can reach high mutation loads within a single generation. High mtDNA copy numbers in mature oocytes are established by mtDNA replication during oocyte development. Bottleneck differences between germline and non-germline cells, due to early differentiation of PGCs, may account for different distribution patterns of familial mutations.http://www.sciencedirect.com/science/article/pii/S221112471630763X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Auke B.C. Otten Tom E.J. Theunissen Josien G. Derhaag Ellen H. Lambrichs Iris B.W. Boesten Marie Winandy Aafke P.A. van Montfoort Katsiaryna Tarbashevich Erez Raz Mike Gerards Jo M. Vanoevelen Bianca J.C. van den Bosch Marc Muller Hubert J.M. Smeets |
spellingShingle |
Auke B.C. Otten Tom E.J. Theunissen Josien G. Derhaag Ellen H. Lambrichs Iris B.W. Boesten Marie Winandy Aafke P.A. van Montfoort Katsiaryna Tarbashevich Erez Raz Mike Gerards Jo M. Vanoevelen Bianca J.C. van den Bosch Marc Muller Hubert J.M. Smeets Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells Cell Reports |
author_facet |
Auke B.C. Otten Tom E.J. Theunissen Josien G. Derhaag Ellen H. Lambrichs Iris B.W. Boesten Marie Winandy Aafke P.A. van Montfoort Katsiaryna Tarbashevich Erez Raz Mike Gerards Jo M. Vanoevelen Bianca J.C. van den Bosch Marc Muller Hubert J.M. Smeets |
author_sort |
Auke B.C. Otten |
title |
Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells |
title_short |
Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells |
title_full |
Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells |
title_fullStr |
Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells |
title_full_unstemmed |
Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells |
title_sort |
differences in strength and timing of the mtdna bottleneck between zebrafish germline and non-germline cells |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2016-07-01 |
description |
We studied the mtDNA bottleneck in zebrafish to elucidate size, timing, and variation in germline and non-germline cells. Mature zebrafish oocytes contain, on average, 19.0 × 106 mtDNA molecules with high variation between oocytes. During embryogenesis, the mtDNA copy number decreases to ∼170 mtDNA molecules per primordial germ cell (PGC), a number similar to that in mammals, and to ∼50 per non-PGC. These occur at the same developmental stage, implying considerable variation in mtDNA copy number in (non-)PGCs of the same female, dictated by variation in the mature oocyte. The presence of oocytes with low mtDNA numbers, if similar in humans, could explain how (de novo) mutations can reach high mutation loads within a single generation. High mtDNA copy numbers in mature oocytes are established by mtDNA replication during oocyte development. Bottleneck differences between germline and non-germline cells, due to early differentiation of PGCs, may account for different distribution patterns of familial mutations. |
url |
http://www.sciencedirect.com/science/article/pii/S221112471630763X |
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