A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes

A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes

We investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes, and analyzed action potential parameters by plotting their frequency distributions. In the both cell lines, the distribution analysis revealed that histogram...

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Main Authors: Fernando López-Redondo, Junko Kurokawa, Fumimasa Nomura, Tomoyuki Kaneko, Tomoyo Hamada, Tetsushi Furukawa, Kenji Yasuda
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Journal of Pharmacological Sciences
Subjects:
iPS cells
Cardiomyocyte
Patch-clamp
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861316300329
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spelling doaj-1be1e75119e5426c85396329488737282020-11-24T23:16:58ZengElsevierJournal of Pharmacological Sciences1347-86132016-06-01131214114510.1016/j.jphs.2016.04.015A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytesFernando López-Redondo0Junko Kurokawa1Fumimasa Nomura2Tomoyuki Kaneko3Tomoyo Hamada4Tetsushi Furukawa5Kenji Yasuda6Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, JapanDepartment of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 113-8510, JapanDepartment of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, JapanDepartment of Frontier Bioscience, Hosei University, Koganei, Tokyo, 184-8584, JapanDepartment of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, JapanDepartment of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 113-8510, JapanDepartment of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, JapanWe investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes, and analyzed action potential parameters by plotting their frequency distributions. In the both cell lines, the distribution analysis revealed that histograms of maximum upstroke velocity showed two subpopulations with similar intersection values. Sub-populations with faster maximum upstroke velocity showed significant prolongation of action potential durations by application of E-4031, whereas others did not, which may be partly due to shallower maximum diastolic potentials. We described electrophysiological and pharmacological properties of stem-cell-derived cardiomyocytes in the respective sub-populations, which provides a way to characterize diverse electrical properties of stem-cell-derived cardiomyocytes systematically.http://www.sciencedirect.com/science/article/pii/S1347861316300329iPS cellsCardiomyocytePatch-clamp
collection DOAJ
language English
format Article
sources DOAJ
author Fernando López-Redondo
Junko Kurokawa
Fumimasa Nomura
Tomoyuki Kaneko
Tomoyo Hamada
Tetsushi Furukawa
Kenji Yasuda
spellingShingle Fernando López-Redondo
Junko Kurokawa
Fumimasa Nomura
Tomoyuki Kaneko
Tomoyo Hamada
Tetsushi Furukawa
Kenji Yasuda
A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
Journal of Pharmacological Sciences
iPS cells
Cardiomyocyte
Patch-clamp
author_facet Fernando López-Redondo
Junko Kurokawa
Fumimasa Nomura
Tomoyuki Kaneko
Tomoyo Hamada
Tetsushi Furukawa
Kenji Yasuda
author_sort Fernando López-Redondo
title A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
title_short A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
title_full A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
title_fullStr A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
title_full_unstemmed A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
title_sort distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2016-06-01
description We investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes, and analyzed action potential parameters by plotting their frequency distributions. In the both cell lines, the distribution analysis revealed that histograms of maximum upstroke velocity showed two subpopulations with similar intersection values. Sub-populations with faster maximum upstroke velocity showed significant prolongation of action potential durations by application of E-4031, whereas others did not, which may be partly due to shallower maximum diastolic potentials. We described electrophysiological and pharmacological properties of stem-cell-derived cardiomyocytes in the respective sub-populations, which provides a way to characterize diverse electrical properties of stem-cell-derived cardiomyocytes systematically.
topic iPS cells
Cardiomyocyte
Patch-clamp
url http://www.sciencedirect.com/science/article/pii/S1347861316300329
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