Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model
Background/Aims: The natural polyphenol resveratrol (RSV) has been shown to ameliorate ischemia/reperfusion (I/R)-induced damage. Therefore, a rat model of I/R-induced AKI equipped with intensive monitoring was utilized to examine direct renal protection by RSV in vivo. Methods: AKI was induced by b...
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2017-12-01
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Series: | Kidney & Blood Pressure Research |
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doaj-1bfa689257ab470a9561d91089e2a4922020-11-25T03:32:27ZengKarger PublishersKidney & Blood Pressure Research1420-40961423-01432017-12-014261090110310.1159/000485606485606Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat ModelAnja BienholzRahel Mae PangHana GuberinaUrsula RauenOliver WitzkeBenjamin WildeFrank PetratThorsten FeldkampAndreas KribbenBackground/Aims: The natural polyphenol resveratrol (RSV) has been shown to ameliorate ischemia/reperfusion (I/R)-induced damage. Therefore, a rat model of I/R-induced AKI equipped with intensive monitoring was utilized to examine direct renal protection by RSV in vivo. Methods: AKI was induced by bilateral renal clamping (45 min) followed by reperfusion (3 h). Solvent-free RSV was continuously infused intravenously (0.056 and 0.28 mg/kg) in a total volume of 7 ml/kg/h starting from 30 min before renal clamping. At a mean arterial blood pressure below 70 mmHg for more than 5 min, bolus injections of 0.5 ml 0.9% NaCl solution were administered repetitively (max. 5 ml/kg/h). Results: No differences could be found between normoxic control groups with/without RSV. Bilateral renal clamping and subsequent reperfusion caused a progressive rise in creatinine, cystatin C, and CK, a decrease in cellular ATP content and diuresis. Infusion of RSV increased sirtuin 1 expression after ischemia/reperfusion and was associated with decreased blood pressure during ischemia and early reperfusion accompanied by an increased requirement of bolus injections as well as with increased expression of TNFα. Conclusion: RSV did not exert protective effects on I/R-induced AKI in the present short-term in vivo rat model. The lack of protection is potentially connected to aggravation of blood pressure instability.https://www.karger.com/Article/FullText/485606Acute kidney injuryIschemia and reperfusion injuryBlood pressureResveratrol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anja Bienholz Rahel Mae Pang Hana Guberina Ursula Rauen Oliver Witzke Benjamin Wilde Frank Petrat Thorsten Feldkamp Andreas Kribben |
spellingShingle |
Anja Bienholz Rahel Mae Pang Hana Guberina Ursula Rauen Oliver Witzke Benjamin Wilde Frank Petrat Thorsten Feldkamp Andreas Kribben Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model Kidney & Blood Pressure Research Acute kidney injury Ischemia and reperfusion injury Blood pressure Resveratrol |
author_facet |
Anja Bienholz Rahel Mae Pang Hana Guberina Ursula Rauen Oliver Witzke Benjamin Wilde Frank Petrat Thorsten Feldkamp Andreas Kribben |
author_sort |
Anja Bienholz |
title |
Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model |
title_short |
Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model |
title_full |
Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model |
title_fullStr |
Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model |
title_full_unstemmed |
Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model |
title_sort |
resveratrol does not protect from ischemia-induced acute kidney injury in an in vivo rat model |
publisher |
Karger Publishers |
series |
Kidney & Blood Pressure Research |
issn |
1420-4096 1423-0143 |
publishDate |
2017-12-01 |
description |
Background/Aims: The natural polyphenol resveratrol (RSV) has been shown to ameliorate ischemia/reperfusion (I/R)-induced damage. Therefore, a rat model of I/R-induced AKI equipped with intensive monitoring was utilized to examine direct renal protection by RSV in vivo. Methods: AKI was induced by bilateral renal clamping (45 min) followed by reperfusion (3 h). Solvent-free RSV was continuously infused intravenously (0.056 and 0.28 mg/kg) in a total volume of 7 ml/kg/h starting from 30 min before renal clamping. At a mean arterial blood pressure below 70 mmHg for more than 5 min, bolus injections of 0.5 ml 0.9% NaCl solution were administered repetitively (max. 5 ml/kg/h). Results: No differences could be found between normoxic control groups with/without RSV. Bilateral renal clamping and subsequent reperfusion caused a progressive rise in creatinine, cystatin C, and CK, a decrease in cellular ATP content and diuresis. Infusion of RSV increased sirtuin 1 expression after ischemia/reperfusion and was associated with decreased blood pressure during ischemia and early reperfusion accompanied by an increased requirement of bolus injections as well as with increased expression of TNFα. Conclusion: RSV did not exert protective effects on I/R-induced AKI in the present short-term in vivo rat model. The lack of protection is potentially connected to aggravation of blood pressure instability. |
topic |
Acute kidney injury Ischemia and reperfusion injury Blood pressure Resveratrol |
url |
https://www.karger.com/Article/FullText/485606 |
work_keys_str_mv |
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