Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats
MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goat...
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Asian-Australasian Association of Animal Production Societies
2013-03-01
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doaj-1c048389a8b94f3c907763657793494e2020-11-24T23:51:48ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172013-03-0126330931510.5713/ajas.2012.124224651Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy GoatsZhibin JiGuizhi WangChunlan ZhangZhijing XieZhaohua LiuJianmin WangMicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats.http://www.ajas.info/upload/pdf/ajas-26-3-309-2.pdfMicroRNAMammary GlandGoatSolexa High-throughput Sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhibin Ji Guizhi Wang Chunlan Zhang Zhijing Xie Zhaohua Liu Jianmin Wang |
spellingShingle |
Zhibin Ji Guizhi Wang Chunlan Zhang Zhijing Xie Zhaohua Liu Jianmin Wang Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats Asian-Australasian Journal of Animal Sciences MicroRNA Mammary Gland Goat Solexa High-throughput Sequencing |
author_facet |
Zhibin Ji Guizhi Wang Chunlan Zhang Zhijing Xie Zhaohua Liu Jianmin Wang |
author_sort |
Zhibin Ji |
title |
Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats |
title_short |
Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats |
title_full |
Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats |
title_fullStr |
Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats |
title_full_unstemmed |
Identification and Function Prediction of Novel MicroRNAs in Laoshan Dairy Goats |
title_sort |
identification and function prediction of novel micrornas in laoshan dairy goats |
publisher |
Asian-Australasian Association of Animal Production Societies |
series |
Asian-Australasian Journal of Animal Sciences |
issn |
1011-2367 1976-5517 |
publishDate |
2013-03-01 |
description |
MicroRNAs are a class of endogenous small RNAs that play important roles in post-transcriptional gene regulation by directing degradation of mRNAs or facilitating repression of target gene translation. In this study, three small RNA cDNA libraries from the mammary gland tissues of Laoshan dairy goats (Capra hircus) were constructed and sequenced, individually. Through Solexa high-throughput sequencing and bioinformatics analysis, we obtained 50 presumptive novel miRNAs candidates, and 55,448 putative target genes were predicted. GO annotations and KEGG pathway analyses showed the majority of target genes were involved in various biological processes and metabolic pathways. Our results discovered more information about the regulation network between miRNAs and mRNAs and paved a foundation for the molecular genetics of mammary gland development in goats. |
topic |
MicroRNA Mammary Gland Goat Solexa High-throughput Sequencing |
url |
http://www.ajas.info/upload/pdf/ajas-26-3-309-2.pdf |
work_keys_str_mv |
AT zhibinji identificationandfunctionpredictionofnovelmicrornasinlaoshandairygoats AT guizhiwang identificationandfunctionpredictionofnovelmicrornasinlaoshandairygoats AT chunlanzhang identificationandfunctionpredictionofnovelmicrornasinlaoshandairygoats AT zhijingxie identificationandfunctionpredictionofnovelmicrornasinlaoshandairygoats AT zhaohualiu identificationandfunctionpredictionofnovelmicrornasinlaoshandairygoats AT jianminwang identificationandfunctionpredictionofnovelmicrornasinlaoshandairygoats |
_version_ |
1725476049590943744 |