A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms
Shiga toxin-producing E. coli (STEC), of various serogroups harboring the intimin gene, form a serious threat to human health. They are asymptomatically carried by cattle. In this study, a quantitative real-time PCR (qPCR) method was developed as a molecular method to detect and quantify Shiga toxin...
Main Authors: | Karen Verstraete, Els Van Coillie, Hadewig Werbrouck, Stephanie Van Weyenberg, Lieve Herman, Jurgen Del-Favero, Peter De Rijk, Lieven De Zutter, Maria-Adelheid Joris, Marc Heyndrickx, Koen DeReu |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2014-03-01
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Series: | Toxins |
Subjects: | |
Online Access: | http://www.mdpi.com/2072-6651/6/4/1201 |
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