The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice
Abstract Wound healing is a complex physiologic process that proceeds in overlapping, sequential steps. Plasminogen promotes fibrinolysis and potentiates the inflammatory response during wound healing. We have tested the hypothesis that the novel plasminogen receptor, Plg-RKT, regulates key steps in...
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2020-12-01
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doaj-1c46c49f28ce42a688b1373f2896d9f32020-12-13T12:03:36ZengNature Publishing GroupCell Death and Disease2041-48892020-12-01111211510.1038/s41419-020-03230-1The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in miceLina Ny0Robert J. Parmer1Yue Shen2Sandra Holmberg3Nagyung Baik4Assar Bäckman5Jessica Broden6Malgorzata Wilczynska7Tor Ny8Lindsey A. Miles9Department of Medical Biochemistry and Biophysics, Umeå UniversityDepartment of Medicine, University of California San DiegoDepartment of Medical Biochemistry and Biophysics, Umeå UniversityOmnio AB Tvistevägen 48Department of Molecular Medicine, The Scripps Research InstituteOmnio AB Tvistevägen 48Omnio AB Tvistevägen 48Department of Medical Biochemistry and Biophysics, Umeå UniversityDepartment of Medical Biochemistry and Biophysics, Umeå UniversityDepartment of Molecular Medicine, The Scripps Research InstituteAbstract Wound healing is a complex physiologic process that proceeds in overlapping, sequential steps. Plasminogen promotes fibrinolysis and potentiates the inflammatory response during wound healing. We have tested the hypothesis that the novel plasminogen receptor, Plg-RKT, regulates key steps in wound healing. Standardized burn wounds were induced in mice and time dependence of wound closure was quantified. Healing in Plg-RKT −/− mice was significantly delayed during the proliferation phase. Expression of inflammatory cytokines was dysregulated in Plg-RKT −/− wound tissue. Consistent with dysregulated cytokine expression, a significant delay in wound healing during the proliferation phase was observed in mice in which Plg-RKT was specifically deleted in myeloid cells. Following wound closure, the epidermal thickness was less in Plg-RKT −/− wound tissue. Paradoxically, deletion of Plg-RKT, specifically in keratinocytes, significantly accelerated the rate of healing during the proliferation phase. Mechanistically, only two genes were upregulated in Plg-RKT −/− compared with Plg-RKT +/+ wound tissue, filaggrin, and caspase 14. Both filaggrin and caspase 14 promote epidermal differentiation and decrease proliferation, consistent with more rapid wound closure and decreased epidermal thickness during the remodeling phase. Fibrin clearance was significantly impaired in Plg-RKT −/− wound tissue. Genetic reduction of fibrinogen levels to 50% completely abrogated the effect of Plg-RKT deletion on the healing of burn wounds. Remarkably, the effects of Plg-RKT deletion on cytokine expression were modulated by reducing fibrinogen levels. In summary, Plg-RKT is a new regulator participating in different phases of cutaneous burn wound healing, which coordinately plays a role in the interrelated responses of inflammation, keratinocyte migration, and fibrinolysis.https://doi.org/10.1038/s41419-020-03230-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lina Ny Robert J. Parmer Yue Shen Sandra Holmberg Nagyung Baik Assar Bäckman Jessica Broden Malgorzata Wilczynska Tor Ny Lindsey A. Miles |
spellingShingle |
Lina Ny Robert J. Parmer Yue Shen Sandra Holmberg Nagyung Baik Assar Bäckman Jessica Broden Malgorzata Wilczynska Tor Ny Lindsey A. Miles The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice Cell Death and Disease |
author_facet |
Lina Ny Robert J. Parmer Yue Shen Sandra Holmberg Nagyung Baik Assar Bäckman Jessica Broden Malgorzata Wilczynska Tor Ny Lindsey A. Miles |
author_sort |
Lina Ny |
title |
The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice |
title_short |
The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice |
title_full |
The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice |
title_fullStr |
The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice |
title_full_unstemmed |
The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice |
title_sort |
plasminogen receptor, plg-rkt, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice |
publisher |
Nature Publishing Group |
series |
Cell Death and Disease |
issn |
2041-4889 |
publishDate |
2020-12-01 |
description |
Abstract Wound healing is a complex physiologic process that proceeds in overlapping, sequential steps. Plasminogen promotes fibrinolysis and potentiates the inflammatory response during wound healing. We have tested the hypothesis that the novel plasminogen receptor, Plg-RKT, regulates key steps in wound healing. Standardized burn wounds were induced in mice and time dependence of wound closure was quantified. Healing in Plg-RKT −/− mice was significantly delayed during the proliferation phase. Expression of inflammatory cytokines was dysregulated in Plg-RKT −/− wound tissue. Consistent with dysregulated cytokine expression, a significant delay in wound healing during the proliferation phase was observed in mice in which Plg-RKT was specifically deleted in myeloid cells. Following wound closure, the epidermal thickness was less in Plg-RKT −/− wound tissue. Paradoxically, deletion of Plg-RKT, specifically in keratinocytes, significantly accelerated the rate of healing during the proliferation phase. Mechanistically, only two genes were upregulated in Plg-RKT −/− compared with Plg-RKT +/+ wound tissue, filaggrin, and caspase 14. Both filaggrin and caspase 14 promote epidermal differentiation and decrease proliferation, consistent with more rapid wound closure and decreased epidermal thickness during the remodeling phase. Fibrin clearance was significantly impaired in Plg-RKT −/− wound tissue. Genetic reduction of fibrinogen levels to 50% completely abrogated the effect of Plg-RKT deletion on the healing of burn wounds. Remarkably, the effects of Plg-RKT deletion on cytokine expression were modulated by reducing fibrinogen levels. In summary, Plg-RKT is a new regulator participating in different phases of cutaneous burn wound healing, which coordinately plays a role in the interrelated responses of inflammation, keratinocyte migration, and fibrinolysis. |
url |
https://doi.org/10.1038/s41419-020-03230-1 |
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