Application of virus-induced gene silencing for identification of FHB resistant genes
Virus-induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus (BSMV)-based VIGS system to evaluate Fusarium head blight (FHB) resi...
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doaj-1c612d6ef0f446c99efcbf0c388465032021-06-08T04:40:43ZengElsevierJournal of Integrative Agriculture2095-31192019-10-01181021832192Application of virus-induced gene silencing for identification of FHB resistant genesYan-hui FAN0Bing-qian HOU1Pei-sen SU2Hong-yan WU3Gui-ping WANG4Ling-rang KONG5Xin MA6Hong-wei WANG7State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R.ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R.ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R.ChinaShandong Agricultural University Fertilizer Science Technology Co., Ltd., Feicheng 271600, P.R.ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R.ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R.ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R.China; Correspondence MA Xin, Tel: +86-538-8249278, Fax: +86-538-8242226State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P.R.China; Correspondence WANG Hong-wei, Tel: +86-538-8249278, Fax: +86-538-8242226Virus-induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus (BSMV)-based VIGS system to evaluate Fusarium head blight (FHB) resistance were explored in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different Fusarium graminearum strains were tested and SF06-1 strain was selected for FHB resistance evaluation. Using this system, TaAOC, TaAOS, and TaOPR3 involved in jasmonic acid (JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing TaAOS in wheat by stable transgenic plants.http://www.sciencedirect.com/science/article/pii/S2095311918621185TriticeaeFusarium head blight (FHB)BSMV-VIGSRNAi |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan-hui FAN Bing-qian HOU Pei-sen SU Hong-yan WU Gui-ping WANG Ling-rang KONG Xin MA Hong-wei WANG |
spellingShingle |
Yan-hui FAN Bing-qian HOU Pei-sen SU Hong-yan WU Gui-ping WANG Ling-rang KONG Xin MA Hong-wei WANG Application of virus-induced gene silencing for identification of FHB resistant genes Journal of Integrative Agriculture Triticeae Fusarium head blight (FHB) BSMV-VIGS RNAi |
author_facet |
Yan-hui FAN Bing-qian HOU Pei-sen SU Hong-yan WU Gui-ping WANG Ling-rang KONG Xin MA Hong-wei WANG |
author_sort |
Yan-hui FAN |
title |
Application of virus-induced gene silencing for identification of FHB resistant genes |
title_short |
Application of virus-induced gene silencing for identification of FHB resistant genes |
title_full |
Application of virus-induced gene silencing for identification of FHB resistant genes |
title_fullStr |
Application of virus-induced gene silencing for identification of FHB resistant genes |
title_full_unstemmed |
Application of virus-induced gene silencing for identification of FHB resistant genes |
title_sort |
application of virus-induced gene silencing for identification of fhb resistant genes |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2019-10-01 |
description |
Virus-induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus (BSMV)-based VIGS system to evaluate Fusarium head blight (FHB) resistance were explored in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different Fusarium graminearum strains were tested and SF06-1 strain was selected for FHB resistance evaluation. Using this system, TaAOC, TaAOS, and TaOPR3 involved in jasmonic acid (JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing TaAOS in wheat by stable transgenic plants. |
topic |
Triticeae Fusarium head blight (FHB) BSMV-VIGS RNAi |
url |
http://www.sciencedirect.com/science/article/pii/S2095311918621185 |
work_keys_str_mv |
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1721390452890927104 |