Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust.
Leaf rust (Puccinia triticina Eriks.) is a fungal disease of wheat (Triticum spp.), which causes considerable yield loss. Adult plant resistance (APR) is one of the most sustainable approaches to control leaf rust. In this study, field-testing was carried out across ten different locations, followed...
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doaj-1c6a8ca30ebf4d14b17cc17f6108d3482021-03-03T20:46:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01143e021346810.1371/journal.pone.0213468Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust.Sundeep KumarB S PhogatV K VikasA K SharmaM S SaharanAmit Kumar SinghJyoti KumariRakesh SinghSherry Rachel JacobG P SinghM SivasamyP JayaprakashM MeetaJ P JaiswalDeep ShikhaB K HonraoI K KalappanavarP C MishraS P SinghS S VaishV A SolankiLeaf rust (Puccinia triticina Eriks.) is a fungal disease of wheat (Triticum spp.), which causes considerable yield loss. Adult plant resistance (APR) is one of the most sustainable approaches to control leaf rust. In this study, field-testing was carried out across ten different locations, followed by molecular screening, to detect the presence of APR genes, Lr34+, Lr46+, Lr67+ and Lr68 in Indian wheat germplasm. In field screening, 190 wheat accessions were selected from 6,319 accessions based on leaf tip necrosis (LTN), disease severity and the average coefficient of infection. Molecular screening revealed that 73% of the accessions possessed known APR genes either as single or as a combination of two or three genes. The occurrence of increased LTN intensity, decreased leaf rust severity and greater expression of APR genes were more in relatively cooler locations. In 52 lines, although the presence of the APR genes was not detected, it still displayed high levels of resistance. Furthermore, 49 accessions possessing either two or three APR genes were evaluated for stability across locations for grain yield. It emerged that eight accessions had wider adaptability. Resistance based on APR genes, in the background of high yielding cultivars, is expected to provide a high level of race non-specific resistance, which is durable.https://doi.org/10.1371/journal.pone.0213468 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sundeep Kumar B S Phogat V K Vikas A K Sharma M S Saharan Amit Kumar Singh Jyoti Kumari Rakesh Singh Sherry Rachel Jacob G P Singh M Sivasamy P Jayaprakash M Meeta J P Jaiswal Deep Shikha B K Honrao I K Kalappanavar P C Mishra S P Singh S S Vaish V A Solanki |
spellingShingle |
Sundeep Kumar B S Phogat V K Vikas A K Sharma M S Saharan Amit Kumar Singh Jyoti Kumari Rakesh Singh Sherry Rachel Jacob G P Singh M Sivasamy P Jayaprakash M Meeta J P Jaiswal Deep Shikha B K Honrao I K Kalappanavar P C Mishra S P Singh S S Vaish V A Solanki Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust. PLoS ONE |
author_facet |
Sundeep Kumar B S Phogat V K Vikas A K Sharma M S Saharan Amit Kumar Singh Jyoti Kumari Rakesh Singh Sherry Rachel Jacob G P Singh M Sivasamy P Jayaprakash M Meeta J P Jaiswal Deep Shikha B K Honrao I K Kalappanavar P C Mishra S P Singh S S Vaish V A Solanki |
author_sort |
Sundeep Kumar |
title |
Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust. |
title_short |
Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust. |
title_full |
Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust. |
title_fullStr |
Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust. |
title_full_unstemmed |
Mining of Indian wheat germplasm collection for adult plant resistance to leaf rust. |
title_sort |
mining of indian wheat germplasm collection for adult plant resistance to leaf rust. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2019-01-01 |
description |
Leaf rust (Puccinia triticina Eriks.) is a fungal disease of wheat (Triticum spp.), which causes considerable yield loss. Adult plant resistance (APR) is one of the most sustainable approaches to control leaf rust. In this study, field-testing was carried out across ten different locations, followed by molecular screening, to detect the presence of APR genes, Lr34+, Lr46+, Lr67+ and Lr68 in Indian wheat germplasm. In field screening, 190 wheat accessions were selected from 6,319 accessions based on leaf tip necrosis (LTN), disease severity and the average coefficient of infection. Molecular screening revealed that 73% of the accessions possessed known APR genes either as single or as a combination of two or three genes. The occurrence of increased LTN intensity, decreased leaf rust severity and greater expression of APR genes were more in relatively cooler locations. In 52 lines, although the presence of the APR genes was not detected, it still displayed high levels of resistance. Furthermore, 49 accessions possessing either two or three APR genes were evaluated for stability across locations for grain yield. It emerged that eight accessions had wider adaptability. Resistance based on APR genes, in the background of high yielding cultivars, is expected to provide a high level of race non-specific resistance, which is durable. |
url |
https://doi.org/10.1371/journal.pone.0213468 |
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