Nanophotonics enhanced coverslip for phase imaging in biology
Abstract The ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experimentally demonstrate a novel nanostructured coverslip that converts phase information to high-contrast intensity images. This compact device enables real-time, all...
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2021-05-01
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Series: | Light: Science & Applications |
Online Access: | https://doi.org/10.1038/s41377-021-00540-7 |
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doaj-1c6c992733834e2a93cf2f722318c1e82021-05-09T11:14:53ZengNature Publishing GroupLight: Science & Applications2047-75382021-05-011011610.1038/s41377-021-00540-7Nanophotonics enhanced coverslip for phase imaging in biologyLukas Wesemann0Jon Rickett1Jingchao Song2Jieqiong Lou3Elizabeth Hinde4Timothy J. Davis5Ann Roberts6School of Physics, University of MelbourneSchool of Physics, University of MelbourneSchool of Physics, University of MelbourneSchool of Physics, University of MelbourneSchool of Physics, University of MelbourneSchool of Physics, University of MelbourneSchool of Physics, University of MelbourneAbstract The ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experimentally demonstrate a novel nanostructured coverslip that converts phase information to high-contrast intensity images. This compact device enables real-time, all-optical generation of pseudo three-dimensional images of phase objects on transmission. We show that by placing unstained human cancer cells on the device, the internal structure within the cells can be clearly seen. Our research demonstrates the significant potential of nanophotonic devices for integration into compact imaging and medical diagnostic devices. The nanophotonics enhanced coverslip (NEC) enables ultra-compact phase imaging of samples placed directly on top of the device. Visualisation of artificial phase objects and unstained biological cells is demonstrated.https://doi.org/10.1038/s41377-021-00540-7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lukas Wesemann Jon Rickett Jingchao Song Jieqiong Lou Elizabeth Hinde Timothy J. Davis Ann Roberts |
spellingShingle |
Lukas Wesemann Jon Rickett Jingchao Song Jieqiong Lou Elizabeth Hinde Timothy J. Davis Ann Roberts Nanophotonics enhanced coverslip for phase imaging in biology Light: Science & Applications |
author_facet |
Lukas Wesemann Jon Rickett Jingchao Song Jieqiong Lou Elizabeth Hinde Timothy J. Davis Ann Roberts |
author_sort |
Lukas Wesemann |
title |
Nanophotonics enhanced coverslip for phase imaging in biology |
title_short |
Nanophotonics enhanced coverslip for phase imaging in biology |
title_full |
Nanophotonics enhanced coverslip for phase imaging in biology |
title_fullStr |
Nanophotonics enhanced coverslip for phase imaging in biology |
title_full_unstemmed |
Nanophotonics enhanced coverslip for phase imaging in biology |
title_sort |
nanophotonics enhanced coverslip for phase imaging in biology |
publisher |
Nature Publishing Group |
series |
Light: Science & Applications |
issn |
2047-7538 |
publishDate |
2021-05-01 |
description |
Abstract The ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experimentally demonstrate a novel nanostructured coverslip that converts phase information to high-contrast intensity images. This compact device enables real-time, all-optical generation of pseudo three-dimensional images of phase objects on transmission. We show that by placing unstained human cancer cells on the device, the internal structure within the cells can be clearly seen. Our research demonstrates the significant potential of nanophotonic devices for integration into compact imaging and medical diagnostic devices. The nanophotonics enhanced coverslip (NEC) enables ultra-compact phase imaging of samples placed directly on top of the device. Visualisation of artificial phase objects and unstained biological cells is demonstrated. |
url |
https://doi.org/10.1038/s41377-021-00540-7 |
work_keys_str_mv |
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