Anti-bacterial effect of marine sea grasses mediated endophytic actinomycetes against K. pneumoniae

In this study, the pure, pale white, powdery nature and pigment produced endophytic actinomycetes strains were isolated from marine Sea grasses sample. The isolated strains were endophytes as confirmed by surface sterilization and validation methods. The anti-bacterial efficacy materials of the crud...

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Bibliographic Details
Main Authors: Khalid S. Almaary, Naiyf S. Alharbi, Shine Kadaikunnan, Jamal M. Khaled, Govindan Rajivgandhi, Govindan Ramachandran, Chelliah Chenthis Kanisha, Manavalan Murugan, Khalid F. Alanzi, Natesan Manoharan
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Journal of King Saud University: Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S1018364721001890
Description
Summary:In this study, the pure, pale white, powdery nature and pigment produced endophytic actinomycetes strains were isolated from marine Sea grasses sample. The isolated strains were endophytes as confirmed by surface sterilization and validation methods. The anti-bacterial efficacy materials of the crude compound were extracted by liquid–liquid extraction using various organic solvents. Anti-bacterial efficacy of the isolated actinomycetes strain RMN 10 was exhibited excellent anti-bacterial activity against K. pneumoniae at increasing concentration. In addition, the minimum inhibition concentration (MIC) of the crude actinomycetes compound has excellent anti-bacterial activity at 250 µg/mL concentration and this concentration was fixed as a MIC. Further, the structural and shape damages of actinomycetes crude compound treated K. pneumoniae cells were clearly identified in SEM images. All the invitro experiments were proved that the actinomycetes compound has excellent anti-bacterial activity against K. pneumoniae.
ISSN:1018-3647