Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions.
The objective of the present study was to investigate the presence of interleukin (IL)-27 in pleural effusions and to evaluate the diagnostic significance of pleural IL-27. The concentrations of IL-27 were determined in pleural fluids and sera from 68 patients with tuberculous pleural effusion, 63 m...
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2012-01-01
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doaj-1c91d46faaf34e6eaf493cbee1288d192020-11-25T01:17:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4045010.1371/journal.pone.0040450Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions.Wei-Bing YangQiu-Li LiangZhi-Jian YeChun-Mi NiuWan-Li MaXian-Zhi XiongRong-Hui DuQiong ZhouJian-Chu ZhangHuan-Zhong ShiThe objective of the present study was to investigate the presence of interleukin (IL)-27 in pleural effusions and to evaluate the diagnostic significance of pleural IL-27. The concentrations of IL-27 were determined in pleural fluids and sera from 68 patients with tuberculous pleural effusion, 63 malignant pleural effusion, 22 infectious pleural effusion, and 21 transudative pleural effusion. Flow cytometry was used to identify which pleural cell types expressed IL-27. It was found that the concentrations of pleural IL-27 in tuberculous group were significantly higher than those in malignant, infectious, and transudative groups, respectively. Pleural CD4(+) T cells, CD8(+) T cells, NK cells, NKT cells, B cells, monocytes, macrophages, and mesothelial cells might be the cell sources for IL-27. IL-27 levels could be used for diagnostic purpose for tuberculous pleural effusion, with the cut off value of 1,007 ng/L, IL-27 had a sensitivity of 92.7% and specificity of 99.1% for differential diagnosing tuberculous pleural effusion from non-tuberculous pleural effusions. Therefore, compared to non-tuberculous pleural effusions, IL-27 appeared to be increased in tuberculous pleural effusion. IL-27 in pleural fluid is a sensitive and specific biomarker for the differential diagnosing tuberculous pleural effusion from pleural effusions with the other causes.http://europepmc.org/articles/PMC3392223?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei-Bing Yang Qiu-Li Liang Zhi-Jian Ye Chun-Mi Niu Wan-Li Ma Xian-Zhi Xiong Rong-Hui Du Qiong Zhou Jian-Chu Zhang Huan-Zhong Shi |
spellingShingle |
Wei-Bing Yang Qiu-Li Liang Zhi-Jian Ye Chun-Mi Niu Wan-Li Ma Xian-Zhi Xiong Rong-Hui Du Qiong Zhou Jian-Chu Zhang Huan-Zhong Shi Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions. PLoS ONE |
author_facet |
Wei-Bing Yang Qiu-Li Liang Zhi-Jian Ye Chun-Mi Niu Wan-Li Ma Xian-Zhi Xiong Rong-Hui Du Qiong Zhou Jian-Chu Zhang Huan-Zhong Shi |
author_sort |
Wei-Bing Yang |
title |
Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions. |
title_short |
Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions. |
title_full |
Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions. |
title_fullStr |
Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions. |
title_full_unstemmed |
Cell origins and diagnostic accuracy of interleukin 27 in pleural effusions. |
title_sort |
cell origins and diagnostic accuracy of interleukin 27 in pleural effusions. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
The objective of the present study was to investigate the presence of interleukin (IL)-27 in pleural effusions and to evaluate the diagnostic significance of pleural IL-27. The concentrations of IL-27 were determined in pleural fluids and sera from 68 patients with tuberculous pleural effusion, 63 malignant pleural effusion, 22 infectious pleural effusion, and 21 transudative pleural effusion. Flow cytometry was used to identify which pleural cell types expressed IL-27. It was found that the concentrations of pleural IL-27 in tuberculous group were significantly higher than those in malignant, infectious, and transudative groups, respectively. Pleural CD4(+) T cells, CD8(+) T cells, NK cells, NKT cells, B cells, monocytes, macrophages, and mesothelial cells might be the cell sources for IL-27. IL-27 levels could be used for diagnostic purpose for tuberculous pleural effusion, with the cut off value of 1,007 ng/L, IL-27 had a sensitivity of 92.7% and specificity of 99.1% for differential diagnosing tuberculous pleural effusion from non-tuberculous pleural effusions. Therefore, compared to non-tuberculous pleural effusions, IL-27 appeared to be increased in tuberculous pleural effusion. IL-27 in pleural fluid is a sensitive and specific biomarker for the differential diagnosing tuberculous pleural effusion from pleural effusions with the other causes. |
url |
http://europepmc.org/articles/PMC3392223?pdf=render |
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