Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expression

Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expression

Nuclear factor erythroid 2-related factor 2 (Nrf2) is an important transcription factor in the regulation of redox homeostasis, which plays a vital role in metabolism, the cell cycle, and cell protection. In this study, the complete cDNA sequence of Nrf2 gene of grass carp was obtained by rapid ampl...

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Main Authors: Junpeng Wang, Jun Xie, Zhihan Tu, Jing Wei, Wenqian Pan, Jing Hu, Yi Zhang, Xiangjun Leng, Xiaoqin Li
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2018-09-01
Series:Aquaculture and Fisheries
Online Access:http://www.sciencedirect.com/science/article/pii/S2468550X17302204
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record_format Article
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language English
format Article
sources DOAJ
author Junpeng Wang
Jun Xie
Zhihan Tu
Jing Wei
Wenqian Pan
Jing Hu
Yi Zhang
Xiangjun Leng
Xiaoqin Li
spellingShingle Junpeng Wang
Jun Xie
Zhihan Tu
Jing Wei
Wenqian Pan
Jing Hu
Yi Zhang
Xiangjun Leng
Xiaoqin Li
Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expression
Aquaculture and Fisheries
author_facet Junpeng Wang
Jun Xie
Zhihan Tu
Jing Wei
Wenqian Pan
Jing Hu
Yi Zhang
Xiangjun Leng
Xiaoqin Li
author_sort Junpeng Wang
title Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expression
title_short Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expression
title_full Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expression
title_fullStr Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expression
title_full_unstemmed Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expression
title_sort cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (nrf2) gene of grass carp (ctenopharyngodon idellus) and the dietary effect of eucommia ulmoides on gene expression
publisher KeAi Communications Co., Ltd.
series Aquaculture and Fisheries
issn 2468-550X
publishDate 2018-09-01
description Nuclear factor erythroid 2-related factor 2 (Nrf2) is an important transcription factor in the regulation of redox homeostasis, which plays a vital role in metabolism, the cell cycle, and cell protection. In this study, the complete cDNA sequence of Nrf2 gene of grass carp was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR), and the characteristics of Nrf2 proteins predicted from gene sequencing were analyzed by bioinformatics methods. Tissue differential expression patterns were also detected by Real-time Fluorescence quantitative PCR (qRT-PCR). Then, Eucommia ulmoides (EU), a traditional Chinese medicine, was added into diet with an inclusion of 2% to feed grass carp for 3 months to investigate the effects on the expression of Nrf2 gene in tissues. The results showed that the full-length of Nrf2 gene of grass carp is 2760 bp, encoding 593 amino acids, and the predicted Nrf2 protein is a hydrophilic and non-secretory protein. Grass carp Nrf2 gene showed higher expression in kidney, gill, liver, spleen, and blood tissues than in other tissues. In the feeding trial, dietary EU significantly promoted the Nrf2 mRNA expression in kidney, gill, and liver tissues after three months feeding. The results suggest that Nrf2 plays an important role in the anti-oxidative capacity of grass carp and its expression is enhanced by dietary EU. Keywords: Grass carp, Nrf2, RACE, Bioinformatics analysis, Eucommia ulmoides
url http://www.sciencedirect.com/science/article/pii/S2468550X17302204
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spelling doaj-1c9a0fab8464480d93347f31365815152021-02-02T00:10:03ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2018-09-0135196203Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2 (Nrf2) gene of grass carp (Ctenopharyngodon idellus) and the dietary effect of Eucommia ulmoides on gene expressionJunpeng Wang0Jun Xie1Zhihan Tu2Jing Wei3Wenqian Pan4Jing Hu5Yi Zhang6Xiangjun Leng7Xiaoqin Li8Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, ChinaChinese Academy of Fishery Sciences, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Wuxi, 214081, ChinaCentre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, ChinaCentre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, ChinaCentre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, ChinaCentre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, ChinaDepartment of Animal Science, Xichang University, Xichang, 615013, ChinaCentre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. 999 Huchenghuan Road, Shanghai Ocean University, Shanghai, 201306, China.Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. 999 Huchenghuan Road, Shanghai Ocean University, Shanghai, 201306, China..Nuclear factor erythroid 2-related factor 2 (Nrf2) is an important transcription factor in the regulation of redox homeostasis, which plays a vital role in metabolism, the cell cycle, and cell protection. In this study, the complete cDNA sequence of Nrf2 gene of grass carp was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR), and the characteristics of Nrf2 proteins predicted from gene sequencing were analyzed by bioinformatics methods. Tissue differential expression patterns were also detected by Real-time Fluorescence quantitative PCR (qRT-PCR). Then, Eucommia ulmoides (EU), a traditional Chinese medicine, was added into diet with an inclusion of 2% to feed grass carp for 3 months to investigate the effects on the expression of Nrf2 gene in tissues. The results showed that the full-length of Nrf2 gene of grass carp is 2760 bp, encoding 593 amino acids, and the predicted Nrf2 protein is a hydrophilic and non-secretory protein. Grass carp Nrf2 gene showed higher expression in kidney, gill, liver, spleen, and blood tissues than in other tissues. In the feeding trial, dietary EU significantly promoted the Nrf2 mRNA expression in kidney, gill, and liver tissues after three months feeding. The results suggest that Nrf2 plays an important role in the anti-oxidative capacity of grass carp and its expression is enhanced by dietary EU. Keywords: Grass carp, Nrf2, RACE, Bioinformatics analysis, Eucommia ulmoideshttp://www.sciencedirect.com/science/article/pii/S2468550X17302204

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