Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae
Analysis of 49 strains of Fusarium langsethiae originating from northern Europe (Russia, Finland, Sweden, UK, Norway, and Latvia) revealed the presence of spontaneous auxotrophic mutants that reflect natural intraspecific diversity. Our investigations detected that 49.0% of F. langsethiae strains we...
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doaj-1d096815a745478db72e927eed7ae89e2020-11-24T21:09:32ZengMDPI AGMicroorganisms2076-26072017-03-01521410.3390/microorganisms5020014microorganisms5020014Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiaeOlga Gavrilova0Anna Skritnika1Tatiana Gagkaeva2All-Russian Institute of Plant Protection (VIZR), St.-Petersburg, Pushkin 196608, RussiaAll-Russian Institute of Plant Protection (VIZR), St.-Petersburg, Pushkin 196608, RussiaAll-Russian Institute of Plant Protection (VIZR), St.-Petersburg, Pushkin 196608, RussiaAnalysis of 49 strains of Fusarium langsethiae originating from northern Europe (Russia, Finland, Sweden, UK, Norway, and Latvia) revealed the presence of spontaneous auxotrophic mutants that reflect natural intraspecific diversity. Our investigations detected that 49.0% of F. langsethiae strains were auxotrophic mutants for biotin, and 8.2% of the strains required thiamine as a growth factor. They failed to grow on vitamin-free media. For both prototrophic and auxotrophic strains, no growth defect was observed in rich organic media. Without essential vitamins, a significant reduction in the growth of the auxotrophic strains results in a decrease of the formation of T-2 toxin and diacetoxyscirpenol. In addition, all analysed F. langsethiae strains were distinguished into two subgroups based on PCR product sizes. According to our results, 26 and 23 strains of F. langsethiae belong to subgroups I and II respectively. We determined that the deletion in the intergenic spacer (IGS) region of the rDNA of F. langsethiae belonging to subgroup II is linked with temperature sensitivity and causes a decrease in strain growth at 30 °C. Four thiamine auxotrophic strains were found in subgroup I, while 21 biotin auxotrophic strains were detected in subgroups II. To the best of our knowledge, the spontaneous mutations in F. langsethiae observed in the present work have not been previously reported.http://www.mdpi.com/2076-2607/5/2/14auxotrophysubgroups of F. langsethiaetemperature sensitivitybiotinthiaminemedianorthern Europe |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Olga Gavrilova Anna Skritnika Tatiana Gagkaeva |
spellingShingle |
Olga Gavrilova Anna Skritnika Tatiana Gagkaeva Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae Microorganisms auxotrophy subgroups of F. langsethiae temperature sensitivity biotin thiamine media northern Europe |
author_facet |
Olga Gavrilova Anna Skritnika Tatiana Gagkaeva |
author_sort |
Olga Gavrilova |
title |
Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae |
title_short |
Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae |
title_full |
Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae |
title_fullStr |
Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae |
title_full_unstemmed |
Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae |
title_sort |
identification and characterization of spontaneous auxotrophic mutants in fusarium langsethiae |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2017-03-01 |
description |
Analysis of 49 strains of Fusarium langsethiae originating from northern Europe (Russia, Finland, Sweden, UK, Norway, and Latvia) revealed the presence of spontaneous auxotrophic mutants that reflect natural intraspecific diversity. Our investigations detected that 49.0% of F. langsethiae strains were auxotrophic mutants for biotin, and 8.2% of the strains required thiamine as a growth factor. They failed to grow on vitamin-free media. For both prototrophic and auxotrophic strains, no growth defect was observed in rich organic media. Without essential vitamins, a significant reduction in the growth of the auxotrophic strains results in a decrease of the formation of T-2 toxin and diacetoxyscirpenol. In addition, all analysed F. langsethiae strains were distinguished into two subgroups based on PCR product sizes. According to our results, 26 and 23 strains of F. langsethiae belong to subgroups I and II respectively. We determined that the deletion in the intergenic spacer (IGS) region of the rDNA of F. langsethiae belonging to subgroup II is linked with temperature sensitivity and causes a decrease in strain growth at 30 °C. Four thiamine auxotrophic strains were found in subgroup I, while 21 biotin auxotrophic strains were detected in subgroups II. To the best of our knowledge, the spontaneous mutations in F. langsethiae observed in the present work have not been previously reported. |
topic |
auxotrophy subgroups of F. langsethiae temperature sensitivity biotin thiamine media northern Europe |
url |
http://www.mdpi.com/2076-2607/5/2/14 |
work_keys_str_mv |
AT olgagavrilova identificationandcharacterizationofspontaneousauxotrophicmutantsinfusariumlangsethiae AT annaskritnika identificationandcharacterizationofspontaneousauxotrophicmutantsinfusariumlangsethiae AT tatianagagkaeva identificationandcharacterizationofspontaneousauxotrophicmutantsinfusariumlangsethiae |
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1716758110176018432 |