Antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of Vernonia amygdalina del. Leaf in Swiss mice
Objective: Vernonia amygdalina (V. amygdalina) leaf is locally employed in the Southern region of Nigeria in the treatment of malari a infection. This study evaluated the in vivo antiplasmodial, antioxidant and immunomodulatory effect of ethanol extract of V. amygdalina leaf. Materials and Methods...
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Mashhad University of Medical Sciences
2016-03-01
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doaj-1d28021389b74d9c8f4c52877c7740ae2020-11-24T23:40:46ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492016-03-01622362475487Antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of Vernonia amygdalina del. Leaf in Swiss miceEhimwenma Omoregie0Anirban Pal1Department of Biochemistry, Faculty of Life Sciences, University of Benin, PMB 1154, Benin City, NigeriaIn vivo Animal Testing Facility, Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, IndiaObjective: Vernonia amygdalina (V. amygdalina) leaf is locally employed in the Southern region of Nigeria in the treatment of malari a infection. This study evaluated the in vivo antiplasmodial, antioxidant and immunomodulatory effect of ethanol extract of V. amygdalina leaf. Materials and Methods: The active principles of the dried leaf were extracted with ethanol. For quality validation, chemical finger-print of the extract was performed through high performance thin layer chromatography (HPTLC). The extract was assessed for antiplasmodial activity by the standard four-day suppressive test on Plasmodium berghei (ANKA) infected male Swiss mice (six weeks old) placed into five groups of six animals each. Result: The absorption spectra from the HPTLC revealed several peaks suggesting presence of some bioactive compounds. Results from the in vivo study showed that the ethanol extract of the plant leaf was significantly active against P. berghei in a dose-dependent manner with the minimum and maximum activity observed in the mice treated orally with 100mg/kg (% inhibition of 23.7%) and 1000 mg/kg (% inhibition of 82.3 %) of the extract, respectively, on day four of the study. There was also a dose-dependent decrease (phttp://ajp.mums.ac.ir/pdf_5487_b156ffd2f2faad1e90f31bf28335c171.htmlAntiplasmodialImmunomodulatoryVernonia amygdalinaPlasmodium bergheiHPTLC |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ehimwenma Omoregie Anirban Pal |
spellingShingle |
Ehimwenma Omoregie Anirban Pal Antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of Vernonia amygdalina del. Leaf in Swiss mice Avicenna Journal of Phytomedicine Antiplasmodial Immunomodulatory Vernonia amygdalina Plasmodium berghei HPTLC |
author_facet |
Ehimwenma Omoregie Anirban Pal |
author_sort |
Ehimwenma Omoregie |
title |
Antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of Vernonia amygdalina del. Leaf in Swiss mice |
title_short |
Antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of Vernonia amygdalina del. Leaf in Swiss mice |
title_full |
Antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of Vernonia amygdalina del. Leaf in Swiss mice |
title_fullStr |
Antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of Vernonia amygdalina del. Leaf in Swiss mice |
title_full_unstemmed |
Antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of Vernonia amygdalina del. Leaf in Swiss mice |
title_sort |
antiplasmodial, antioxidant and immunomodulatory activities of ethanol extract of vernonia amygdalina del. leaf in swiss mice |
publisher |
Mashhad University of Medical Sciences |
series |
Avicenna Journal of Phytomedicine |
issn |
2228-7930 2228-7949 |
publishDate |
2016-03-01 |
description |
Objective: Vernonia amygdalina (V. amygdalina) leaf is locally employed in the Southern region of Nigeria in the treatment of malari a infection. This study evaluated the in vivo antiplasmodial, antioxidant and immunomodulatory effect of ethanol extract of V. amygdalina leaf. Materials and Methods: The active principles of the dried leaf were extracted with ethanol. For quality validation, chemical finger-print of the extract was performed through high performance thin layer chromatography (HPTLC). The extract was assessed for antiplasmodial activity by the standard four-day suppressive test on Plasmodium berghei (ANKA) infected male Swiss mice (six weeks old) placed into five groups of six animals each. Result: The absorption spectra from the HPTLC revealed several peaks suggesting presence of some bioactive compounds. Results from the in vivo study showed that the ethanol extract of the plant leaf was significantly active against P. berghei in a dose-dependent manner with the minimum and maximum activity observed in the mice treated orally with 100mg/kg (% inhibition of 23.7%) and 1000 mg/kg (% inhibition of 82.3 %) of the extract, respectively, on day four of the study. There was also a dose-dependent decrease (p |
topic |
Antiplasmodial Immunomodulatory Vernonia amygdalina Plasmodium berghei HPTLC |
url |
http://ajp.mums.ac.ir/pdf_5487_b156ffd2f2faad1e90f31bf28335c171.html |
work_keys_str_mv |
AT ehimwenmaomoregie antiplasmodialantioxidantandimmunomodulatoryactivitiesofethanolextractofvernoniaamygdalinadelleafinswissmice AT anirbanpal antiplasmodialantioxidantandimmunomodulatoryactivitiesofethanolextractofvernoniaamygdalinadelleafinswissmice |
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