ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources
Recently, Pseudomonas aeruginosa infections proportions have increased significantly. Molecular typing and virulence analysis are good techniques, which can lead us to know P. aeruginosa infections. P. aeruginosa isolates were identified by using molecular method (16S rDNA gene) via PCR technique fo...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | Arabic |
Published: |
Al-Mustansiriyah University
2018-11-01
|
Series: | Mustansiriyah Journal of Science |
Subjects: | |
Online Access: | http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/345 |
id |
doaj-1d2fc114420648c7ad08ae7ba2cda6b6 |
---|---|
record_format |
Article |
spelling |
doaj-1d2fc114420648c7ad08ae7ba2cda6b62020-11-25T02:09:17ZaraAl-Mustansiriyah UniversityMustansiriyah Journal of Science1814-635X2521-35202018-11-01292506210.23851/mjs.v29i2.345179ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sourcesruqaia sabbar sallman0Suzan Saadi Hussein1Munum Radwan Ali2Department of Biology, College of Science, Mustansiriyah UniversityDepartment of Biology, College of Science, Mustansiriyah University, IRAQ.Department of Biology, College of Science, Mustansiriyah University, IRAQ.Recently, Pseudomonas aeruginosa infections proportions have increased significantly. Molecular typing and virulence analysis are good techniques, which can lead us to know P. aeruginosa infections. P. aeruginosa isolates were identified by using molecular method (16S rDNA gene) via PCR technique for accurate identification. The highest percent 41.26% of P. aeruginosa bacteria was found in the burn infections followed by 28.57% in wound swabs, 17.46% in ear discharge and lowest percentage were obtained from sputum samples. All isolates classified into six groups (A-F) according to classes of antibiotics. Of the 63 bacterial isolates, 100% were resistant to carbencillin, whereas 31.74% were resistant to ticarcillin and all isolates susceptible to imipenem. In addition all of clinical isolates indicated multidrug resistant (MDR) patterns, the highest rate of MDR was observed with pattern C these isolates were able to resist (9-12) antibiotics. All isolates were typed genotypically by using two methods of amplification, ERIC and RAPD-PCR. The results of the ERIC-PCR typing of P. aeruginosa bacteria that 96.82% showed amplification bands ERIC-PCR also revealed 17 groups of genotypes (A-R) and 4 unique isolates. The results of RAPD-PCR fingerprint revealed 12 groups of genotypes (A-M) of 40–90% similarity according to coefficient values and 4 unique isolates, except 7.93% were untypeble. QS genes (lasI, lasR, rhlI, rhlR), screen showed all isolates 100% were positive for one or more QS genes, in the other hand 82.53% carrying lasI, lasR, rhlI, and rhlR, while the 15.87% carrying lasI, rhlI, and rhlR and 1.58% carrying lasI, lasR, and rhlR genes. ERIC genotyping significantly correlated resistance patterns but not with virulence control QS genes. RAPD genotyping significantly correlated with source of infection, resistance patterns and virulence control QS genes. These results can help initial diagnosis MDR P. aeruginosa outbreaks associated with specific genotyping patterns.http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/345Pseudomonas aeruginosa, ERIC-PCR typing, RAPD-PCR fingerprinting, Quorum sensing genes. |
collection |
DOAJ |
language |
Arabic |
format |
Article |
sources |
DOAJ |
author |
ruqaia sabbar sallman Suzan Saadi Hussein Munum Radwan Ali |
spellingShingle |
ruqaia sabbar sallman Suzan Saadi Hussein Munum Radwan Ali ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources Mustansiriyah Journal of Science Pseudomonas aeruginosa, ERIC-PCR typing, RAPD-PCR fingerprinting, Quorum sensing genes. |
author_facet |
ruqaia sabbar sallman Suzan Saadi Hussein Munum Radwan Ali |
author_sort |
ruqaia sabbar sallman |
title |
ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources |
title_short |
ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources |
title_full |
ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources |
title_fullStr |
ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources |
title_full_unstemmed |
ERIC- PCR Typing, RAPD-PCR Fingerprinting and Quorum Sensing Gene Analysis of Pseudomonas aeruginosa Isolated from Different Clinical Sources |
title_sort |
eric- pcr typing, rapd-pcr fingerprinting and quorum sensing gene analysis of pseudomonas aeruginosa isolated from different clinical sources |
publisher |
Al-Mustansiriyah University |
series |
Mustansiriyah Journal of Science |
issn |
1814-635X 2521-3520 |
publishDate |
2018-11-01 |
description |
Recently, Pseudomonas aeruginosa infections proportions have increased significantly. Molecular typing and virulence analysis are good techniques, which can lead us to know P. aeruginosa infections. P. aeruginosa isolates were identified by using molecular method (16S rDNA gene) via PCR technique for accurate identification. The highest percent 41.26% of P. aeruginosa bacteria was found in the burn infections followed by 28.57% in wound swabs, 17.46% in ear discharge and lowest percentage were obtained from sputum samples. All isolates classified into six groups (A-F) according to classes of antibiotics. Of the 63 bacterial isolates, 100% were resistant to carbencillin, whereas 31.74% were resistant to ticarcillin and all isolates susceptible to imipenem. In addition all of clinical isolates indicated multidrug resistant (MDR) patterns, the highest rate of MDR was observed with pattern C these isolates were able to resist (9-12) antibiotics. All isolates were typed genotypically by using two methods of amplification, ERIC and RAPD-PCR. The results of the ERIC-PCR typing of P. aeruginosa bacteria that 96.82% showed amplification bands ERIC-PCR also revealed 17 groups of genotypes (A-R) and 4 unique isolates. The results of RAPD-PCR fingerprint revealed 12 groups of genotypes (A-M) of 40–90% similarity according to coefficient values and 4 unique isolates, except 7.93% were untypeble. QS genes (lasI, lasR, rhlI, rhlR), screen showed all isolates 100% were positive for one or more QS genes, in the other hand 82.53% carrying lasI, lasR, rhlI, and rhlR, while the 15.87% carrying lasI, rhlI, and rhlR and 1.58% carrying lasI, lasR, and rhlR genes. ERIC genotyping significantly correlated resistance patterns but not with virulence control QS genes. RAPD genotyping significantly correlated with source of infection, resistance patterns and virulence control QS genes. These results can help initial diagnosis MDR P. aeruginosa outbreaks associated with specific genotyping patterns. |
topic |
Pseudomonas aeruginosa, ERIC-PCR typing, RAPD-PCR fingerprinting, Quorum sensing genes. |
url |
http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/345 |
work_keys_str_mv |
AT ruqaiasabbarsallman ericpcrtypingrapdpcrfingerprintingandquorumsensinggeneanalysisofpseudomonasaeruginosaisolatedfromdifferentclinicalsources AT suzansaadihussein ericpcrtypingrapdpcrfingerprintingandquorumsensinggeneanalysisofpseudomonasaeruginosaisolatedfromdifferentclinicalsources AT munumradwanali ericpcrtypingrapdpcrfingerprintingandquorumsensinggeneanalysisofpseudomonasaeruginosaisolatedfromdifferentclinicalsources |
_version_ |
1724924726840655872 |