Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging
<p>Abstract</p> <p>Background</p> <p>Application of superparamagnetic iron oxide nanoparticles (SPIOs) as the contrast agent has improved the quality of magnetic resonance (MR) imaging. Low efficiency of loading the commercially available iron oxide nanoparticles into c...
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doaj-1d34f9c0f89f4080a92576e59ceaf02d2020-11-24T22:07:15ZengBMCJournal of Biomedical Science1021-77701423-01272009-09-011618610.1186/1423-0127-16-86Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imagingMou Chung-YuanChen Jyh-HorngLin Chung-WuChen Chao-YuChang KarenChang Po-YuanHuang Huey-ChungChang Zee-FenChang Fu-Hsiung<p>Abstract</p> <p>Background</p> <p>Application of superparamagnetic iron oxide nanoparticles (SPIOs) as the contrast agent has improved the quality of magnetic resonance (MR) imaging. Low efficiency of loading the commercially available iron oxide nanoparticles into cells and the cytotoxicity of previously formulated complexes limit their usage as the image probe. Here, we formulated new cationic lipid nanoparticles containing SPIOs feasible for <it>in vivo </it>imaging.</p> <p>Methods</p> <p>Hydrophobic SPIOs were incorporated into cationic lipid 1,2-dioleoyl-3-(trimethylammonium) propane (DOTAP) and polyethylene-glycol-2000-1,2-distearyl-3-sn-phosphatidylethanolamine (PEG-DSPE) based micelles by self-assembly procedure to form lipid-coated SPIOs (L-SPIOs). Trace amount of Rhodamine-dioleoyl-phosphatidylethanolamine (Rhodamine-DOPE) was added as a fluorescent indicator. Particle size and zeta potential of L-SPIOs were determined by Dynamic Light Scattering (DLS) and Laser Doppler Velocimetry (LDV), respectively. HeLa, PC-3 and Neuro-2a cells were tested for loading efficiency and cytotoxicity of L-SPIOs using fluorescent microscopy, Prussian blue staining and flow cytometry. L-SPIO-loaded CT-26 cells were tested for <it>in vivo </it>MR imaging.</p> <p>Results</p> <p>The novel formulation generates L-SPIOs particle with the average size of 46 nm. We showed efficient cellular uptake of these L-SPIOs with cationic surface charge into HeLa, PC-3 and Neuro-2a cells. The L-SPIO-loaded cells exhibited similar growth potential as compared to unloaded cells, and could be sorted by a magnet stand over ten-day duration. Furthermore, when SPIO-loaded CT-26 tumor cells were injected into Balb/c mice, the growth status of these tumor cells could be monitored using optical and MR images.</p> <p>Conclusion</p> <p>We have developed a novel cationic lipid-based nanoparticle of SPIOs with high loading efficiency, low cytotoxicity and long-term imaging signals. The results suggested these newly formulated non-toxic lipid-coated magnetic nanoparticles as a versatile image probe for cell tracking.</p> http://www.jbiomedsci.com/content/16/1/86 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mou Chung-Yuan Chen Jyh-Horng Lin Chung-Wu Chen Chao-Yu Chang Karen Chang Po-Yuan Huang Huey-Chung Chang Zee-Fen Chang Fu-Hsiung |
spellingShingle |
Mou Chung-Yuan Chen Jyh-Horng Lin Chung-Wu Chen Chao-Yu Chang Karen Chang Po-Yuan Huang Huey-Chung Chang Zee-Fen Chang Fu-Hsiung Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging Journal of Biomedical Science |
author_facet |
Mou Chung-Yuan Chen Jyh-Horng Lin Chung-Wu Chen Chao-Yu Chang Karen Chang Po-Yuan Huang Huey-Chung Chang Zee-Fen Chang Fu-Hsiung |
author_sort |
Mou Chung-Yuan |
title |
Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging |
title_short |
Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging |
title_full |
Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging |
title_fullStr |
Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging |
title_full_unstemmed |
Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging |
title_sort |
formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging |
publisher |
BMC |
series |
Journal of Biomedical Science |
issn |
1021-7770 1423-0127 |
publishDate |
2009-09-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Application of superparamagnetic iron oxide nanoparticles (SPIOs) as the contrast agent has improved the quality of magnetic resonance (MR) imaging. Low efficiency of loading the commercially available iron oxide nanoparticles into cells and the cytotoxicity of previously formulated complexes limit their usage as the image probe. Here, we formulated new cationic lipid nanoparticles containing SPIOs feasible for <it>in vivo </it>imaging.</p> <p>Methods</p> <p>Hydrophobic SPIOs were incorporated into cationic lipid 1,2-dioleoyl-3-(trimethylammonium) propane (DOTAP) and polyethylene-glycol-2000-1,2-distearyl-3-sn-phosphatidylethanolamine (PEG-DSPE) based micelles by self-assembly procedure to form lipid-coated SPIOs (L-SPIOs). Trace amount of Rhodamine-dioleoyl-phosphatidylethanolamine (Rhodamine-DOPE) was added as a fluorescent indicator. Particle size and zeta potential of L-SPIOs were determined by Dynamic Light Scattering (DLS) and Laser Doppler Velocimetry (LDV), respectively. HeLa, PC-3 and Neuro-2a cells were tested for loading efficiency and cytotoxicity of L-SPIOs using fluorescent microscopy, Prussian blue staining and flow cytometry. L-SPIO-loaded CT-26 cells were tested for <it>in vivo </it>MR imaging.</p> <p>Results</p> <p>The novel formulation generates L-SPIOs particle with the average size of 46 nm. We showed efficient cellular uptake of these L-SPIOs with cationic surface charge into HeLa, PC-3 and Neuro-2a cells. The L-SPIO-loaded cells exhibited similar growth potential as compared to unloaded cells, and could be sorted by a magnet stand over ten-day duration. Furthermore, when SPIO-loaded CT-26 tumor cells were injected into Balb/c mice, the growth status of these tumor cells could be monitored using optical and MR images.</p> <p>Conclusion</p> <p>We have developed a novel cationic lipid-based nanoparticle of SPIOs with high loading efficiency, low cytotoxicity and long-term imaging signals. The results suggested these newly formulated non-toxic lipid-coated magnetic nanoparticles as a versatile image probe for cell tracking.</p> |
url |
http://www.jbiomedsci.com/content/16/1/86 |
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