Evaluation of serial crystallographic structure determination within megahertz pulse trains
The new European X-ray Free-Electron Laser (European XFEL) is the first X-ray free-electron laser capable of delivering intense X-ray pulses with a megahertz interpulse spacing in a wavelength range suitable for atomic resolution structure determination. An outstanding but crucial question is whethe...
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC and ACA
2019-11-01
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| Series: | Structural Dynamics |
| Online Access: | http://dx.doi.org/10.1063/1.5124387 |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Oleksandr Yefanov Dominik Oberthür Richard Bean Max O. Wiedorn Juraj Knoska Gisel Pena Salah Awel Lars Gumprecht Martin Domaracky Iosifina Sarrou P. Lourdu Xavier Markus Metz Saša Bajt Valerio Mariani Yaroslav Gevorkov Thomas A. White Aleksandra Tolstikova Pablo Villanueva-Perez Carolin Seuring Steve Aplin Armando D. Estillore Jochen Küpper Alexander Klyuev Manuela Kuhn Torsten Laurus Heinz Graafsma Diana C. F. Monteiro Martin Trebbin Filipe R. N. C. Maia Francisco Cruz-Mazo Alfonso M. Gañán-Calvo Michael Heymann Connie Darmanin Brian Abbey Marius Schmidt Petra Fromme Klaus Giewekemeyer Marcin Sikorski Rita Graceffa Patrik Vagovic Thomas Kluyver Martin Bergemann Hans Fangohr Jolanta Sztuk-Dambietz Steffen Hauf Natascha Raab Valerii Bondar Adrian P. Mancuso Henry Chapman Anton Barty |
| spellingShingle |
Oleksandr Yefanov Dominik Oberthür Richard Bean Max O. Wiedorn Juraj Knoska Gisel Pena Salah Awel Lars Gumprecht Martin Domaracky Iosifina Sarrou P. Lourdu Xavier Markus Metz Saša Bajt Valerio Mariani Yaroslav Gevorkov Thomas A. White Aleksandra Tolstikova Pablo Villanueva-Perez Carolin Seuring Steve Aplin Armando D. Estillore Jochen Küpper Alexander Klyuev Manuela Kuhn Torsten Laurus Heinz Graafsma Diana C. F. Monteiro Martin Trebbin Filipe R. N. C. Maia Francisco Cruz-Mazo Alfonso M. Gañán-Calvo Michael Heymann Connie Darmanin Brian Abbey Marius Schmidt Petra Fromme Klaus Giewekemeyer Marcin Sikorski Rita Graceffa Patrik Vagovic Thomas Kluyver Martin Bergemann Hans Fangohr Jolanta Sztuk-Dambietz Steffen Hauf Natascha Raab Valerii Bondar Adrian P. Mancuso Henry Chapman Anton Barty Evaluation of serial crystallographic structure determination within megahertz pulse trains Structural Dynamics |
| author_facet |
Oleksandr Yefanov Dominik Oberthür Richard Bean Max O. Wiedorn Juraj Knoska Gisel Pena Salah Awel Lars Gumprecht Martin Domaracky Iosifina Sarrou P. Lourdu Xavier Markus Metz Saša Bajt Valerio Mariani Yaroslav Gevorkov Thomas A. White Aleksandra Tolstikova Pablo Villanueva-Perez Carolin Seuring Steve Aplin Armando D. Estillore Jochen Küpper Alexander Klyuev Manuela Kuhn Torsten Laurus Heinz Graafsma Diana C. F. Monteiro Martin Trebbin Filipe R. N. C. Maia Francisco Cruz-Mazo Alfonso M. Gañán-Calvo Michael Heymann Connie Darmanin Brian Abbey Marius Schmidt Petra Fromme Klaus Giewekemeyer Marcin Sikorski Rita Graceffa Patrik Vagovic Thomas Kluyver Martin Bergemann Hans Fangohr Jolanta Sztuk-Dambietz Steffen Hauf Natascha Raab Valerii Bondar Adrian P. Mancuso Henry Chapman Anton Barty |
| author_sort |
Oleksandr Yefanov |
| title |
Evaluation of serial crystallographic structure determination within megahertz pulse trains |
| title_short |
Evaluation of serial crystallographic structure determination within megahertz pulse trains |
| title_full |
Evaluation of serial crystallographic structure determination within megahertz pulse trains |
| title_fullStr |
Evaluation of serial crystallographic structure determination within megahertz pulse trains |
| title_full_unstemmed |
Evaluation of serial crystallographic structure determination within megahertz pulse trains |
| title_sort |
evaluation of serial crystallographic structure determination within megahertz pulse trains |
| publisher |
AIP Publishing LLC and ACA |
| series |
Structural Dynamics |
| issn |
2329-7778 |
| publishDate |
2019-11-01 |
| description |
The new European X-ray Free-Electron Laser (European XFEL) is the first X-ray free-electron laser capable of delivering intense X-ray pulses with a megahertz interpulse spacing in a wavelength range suitable for atomic resolution structure determination. An outstanding but crucial question is whether the use of a pulse repetition rate nearly four orders of magnitude higher than previously possible results in unwanted structural changes due to either radiation damage or systematic effects on data quality. Here, separate structures from the first and subsequent pulses in the European XFEL pulse train were determined, showing that there is essentially no difference between structures determined from different pulses under currently available operating conditions at the European XFEL. |
| url |
http://dx.doi.org/10.1063/1.5124387 |
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doaj-1d3f433eb9564962815e2362edce9ca42020-11-25T02:48:57ZengAIP Publishing LLC and ACAStructural Dynamics2329-77782019-11-0166064702064702-1010.1063/1.5124387Evaluation of serial crystallographic structure determination within megahertz pulse trainsOleksandr Yefanov0Dominik Oberthür1Richard Bean2Max O. Wiedorn3Juraj Knoska4Gisel Pena5Salah Awel6Lars Gumprecht7Martin Domaracky8Iosifina Sarrou9P. Lourdu Xavier10Markus Metz11Saša Bajt12Valerio Mariani13Yaroslav Gevorkov14Thomas A. White15Aleksandra Tolstikova16Pablo Villanueva-Perez17Carolin Seuring18Steve Aplin19Armando D. Estillore20Jochen Küpper21Alexander Klyuev22Manuela Kuhn23Torsten Laurus24Heinz Graafsma25Diana C. F. Monteiro26Martin Trebbin27Filipe R. N. C. Maia28Francisco Cruz-Mazo29Alfonso M. Gañán-Calvo30Michael Heymann31Connie Darmanin32Brian Abbey33Marius Schmidt34Petra Fromme35Klaus Giewekemeyer36Marcin Sikorski37Rita Graceffa38Patrik Vagovic39Thomas Kluyver40Martin Bergemann41Hans Fangohr42Jolanta Sztuk-Dambietz43Steffen Hauf44Natascha Raab45Valerii Bondar46Adrian P. Mancuso47Henry Chapman48Anton Barty49 Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany Deutsches Elektronen Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany Department of Chemistry, University at Buffalo, 359 Natural Sciences Complex, Buffalo, New York 14260, USA Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092 Sevilla, Spain Dept. de Ingeniería Aeroespacial y Mecánica de Fluidos, ETSI, Universidad de Sevilla, 41092 Sevilla, Spain Intelligent Biointegrative Systems Group, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, D-70569 Stuttgart, Germany ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Victoria 3086, Australia ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Victoria 3086, Australia Physics Department, University of Wisconsin-Milwaukee, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona 85287-1604, USA European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany Center for Free-Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, GermanyThe new European X-ray Free-Electron Laser (European XFEL) is the first X-ray free-electron laser capable of delivering intense X-ray pulses with a megahertz interpulse spacing in a wavelength range suitable for atomic resolution structure determination. An outstanding but crucial question is whether the use of a pulse repetition rate nearly four orders of magnitude higher than previously possible results in unwanted structural changes due to either radiation damage or systematic effects on data quality. Here, separate structures from the first and subsequent pulses in the European XFEL pulse train were determined, showing that there is essentially no difference between structures determined from different pulses under currently available operating conditions at the European XFEL.http://dx.doi.org/10.1063/1.5124387 |