Expression of Low Molecular Mass Proteins in Stone Quarry Workers
Background: In India, there are many unauthorized stone quarries in which millions of workers exposed to crystalline silica dust. Silica dust is the most significant causative agent for the irreversible but preventable disease like silicosis. It is an unavoidable occupational disease among mining...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Pvt. Ltd.
2013-01-01
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| Series: | National Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | http://www.njlm.net/articles/PDF/1964/3-%205158_F(H)_PF1(PH)_PFA(H)_PF1(VH).pdf |
| Summary: | Background: In India, there are many unauthorized stone
quarries in which millions of workers exposed to crystalline silica dust. Silica dust is the most significant causative
agent for the irreversible but preventable disease like silicosis. It is an unavoidable occupational disease among mining
population. Silicosis, silicotuberculosis, chronic bronchitis,
Chronic Obstructive Pulmonary Disease (COPD) and lung
cancer are reported in long term occupational exposure to
silica dust. Early health care is the key to silicosis prevention, but until now no effective indicators of early health care
is developed.
Objectives: The present study was performed on stone
quarry workers for the evaluation of protein fractions by
SDS-PAGE electrophoresis.
Materials and Methods: For this study, Stone quarry
(n=30) workers were selected from the Panchgaon- kuhifata located in central India as experimental subjects with an
exposure of 0-35 years. For comparison, control subjects
(n=20) belonging to the same socio-economic status and
age group were selected. Blood samples were collected.
Separated serum samples were used for SDS-PAGE.
Results: The molecular mass of differentially expressed
protein was determined by log graph method which was
found to be 10 kDa. Remarkably 90% expression of 10 kDa
protein were observed in control subjects but no such expression were determined in serum of stone quarry workers.
Conclusions: The loss of expression of targeted (10 kDa)
protein in stone quarry workers may be used as a potential
peripheral marker for early silica toxicity |
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| ISSN: | 2277-8551 2455-6882 |