Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays
Intensity- and amplitude-weighted average lifetimes, denoted as τI and τA hereafter, are useful indicators for revealing Förster resonance energy transfer (FRET) or fluorescence quenching behaviors. In this work, we discussed the differences between τI and τA and presented several model-free lifetim...
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doaj-1d4d8ec4f636431d8e668ac6ed971d2c2020-11-25T03:53:25ZengFrontiers Media S.A.Frontiers in Physics2296-424X2020-10-01810.3389/fphy.2020.576862576862Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential DecaysYahui Li0Yahui Li1Yahui Li2Sapermsap Natakorn3Yu Chen4Mohammed Safar5Margaret Cunningham6Jinshou Tian7Jinshou Tian8David Day-Uei Li9Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United KingdomKey Laboratory of Ultra-fast Photoelectric Diagnostics Technology, Xi'an Institute of Optics and Precision Mechanics, Xi'an, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaDepartment of Physics, Scottish Universities Physics Alliance, University of Strathclyde, Glasgow, United KingdomDepartment of Physics, Scottish Universities Physics Alliance, University of Strathclyde, Glasgow, United KingdomStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United KingdomStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United KingdomKey Laboratory of Ultra-fast Photoelectric Diagnostics Technology, Xi'an Institute of Optics and Precision Mechanics, Xi'an, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United KingdomIntensity- and amplitude-weighted average lifetimes, denoted as τI and τA hereafter, are useful indicators for revealing Förster resonance energy transfer (FRET) or fluorescence quenching behaviors. In this work, we discussed the differences between τI and τA and presented several model-free lifetime determination algorithms (LDA), including the center-of-mass, phasor, and integral equation methods for fast τI and τA estimations. For model-based LDAs, we discussed the model-mismatch problems, and the results suggest that a bi-exponential model can well approximate a signal following a multi-exponential model. Depending on the application requirements, suggestions about the LDAs to be used are given. The instrument responses of the imaging systems were included in the analysis. We explained why only using the τI model for FRET analysis can be misleading; both τI and τA models should be considered. We also proposed using τA/τI as a new indicator on two-photon fluorescence lifetime images, and the results show that τA/τI is an intuitive tool for visualizing multi-exponential decays.https://www.frontiersin.org/article/10.3389/fphy.2020.576862/fullfluorescence lifetime imaginglifetime determination algorithmaverage lifetimesmulti-exponential decayslifetime image visualizationFRET—fluorescence resonance energy transfer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yahui Li Yahui Li Yahui Li Sapermsap Natakorn Yu Chen Mohammed Safar Margaret Cunningham Jinshou Tian Jinshou Tian David Day-Uei Li |
spellingShingle |
Yahui Li Yahui Li Yahui Li Sapermsap Natakorn Yu Chen Mohammed Safar Margaret Cunningham Jinshou Tian Jinshou Tian David Day-Uei Li Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays Frontiers in Physics fluorescence lifetime imaging lifetime determination algorithm average lifetimes multi-exponential decays lifetime image visualization FRET—fluorescence resonance energy transfer |
author_facet |
Yahui Li Yahui Li Yahui Li Sapermsap Natakorn Yu Chen Mohammed Safar Margaret Cunningham Jinshou Tian Jinshou Tian David Day-Uei Li |
author_sort |
Yahui Li |
title |
Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays |
title_short |
Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays |
title_full |
Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays |
title_fullStr |
Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays |
title_full_unstemmed |
Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays |
title_sort |
investigations on average fluorescence lifetimes for visualizing multi-exponential decays |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physics |
issn |
2296-424X |
publishDate |
2020-10-01 |
description |
Intensity- and amplitude-weighted average lifetimes, denoted as τI and τA hereafter, are useful indicators for revealing Förster resonance energy transfer (FRET) or fluorescence quenching behaviors. In this work, we discussed the differences between τI and τA and presented several model-free lifetime determination algorithms (LDA), including the center-of-mass, phasor, and integral equation methods for fast τI and τA estimations. For model-based LDAs, we discussed the model-mismatch problems, and the results suggest that a bi-exponential model can well approximate a signal following a multi-exponential model. Depending on the application requirements, suggestions about the LDAs to be used are given. The instrument responses of the imaging systems were included in the analysis. We explained why only using the τI model for FRET analysis can be misleading; both τI and τA models should be considered. We also proposed using τA/τI as a new indicator on two-photon fluorescence lifetime images, and the results show that τA/τI is an intuitive tool for visualizing multi-exponential decays. |
topic |
fluorescence lifetime imaging lifetime determination algorithm average lifetimes multi-exponential decays lifetime image visualization FRET—fluorescence resonance energy transfer |
url |
https://www.frontiersin.org/article/10.3389/fphy.2020.576862/full |
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