Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species

Pathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treat...

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Bibliographic Details
Main Authors: Anvitha Hansoge, Sudheesh S Nair, M V Chinnu, M.K. Narayanan, K.D. John Martin
Format: Article
Language:English
Published: Director of Academics and Research, Kerala Veterinary and Animal Sciences University 2021-06-01
Series:Journal of Veterinary and Animal Sciences
Subjects:
Online Access:https://www.jvas.in/public_html/upload/article_file/article_file_qvr8z4.pdf?t=qvr8z4
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Summary:Pathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treatment and prevention protocols. Four cases of chronic obstructive urolithiasis in male goats presented to University Veterinary Hospital were surgically managed by tube cystotomy. The calculi extracted from the bladder were subjected to Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) to identify the composition of the calculi. The infrared wavelength bandwidths of H-O-H stretching vibrations, H-O-H bending modes of vibrations of water molecules, N-H symmetric stretching vibrations, N-H bending vibration and N-H asymmetric bending vibration in NH4+ unit’s ionic phosphate were compared with the available reference spectrum of wavelength and were found to be identical and comparable with the standard infrared wavelength of struvite calculi.
ISSN:0971-0701
2582-0605